https://hdl.handle.net/10259/5086 2026-06-26T07:14:27Z https://hdl.handle.net/10259/11876 How reliable is the evaluation of DNA binding constants? Insights and best practices based on an inter-laboratory fluorescence titration study Dömötör, Orsolya; Binacchi, Francesca; Ribeiro, Nádia; Busto Vázquez, Natalia; González García, Jorge .; García España, Enrique; Correia, Isabel; Enyedy, Éva A.; Hamacek, Josef; Terenzi, Alessio; Basílio, Nuno; Barone, Giampaolo; Cavaco, Isabel; Biver, Tarita In all experimental sciences, the precision and reliability of quantitative measurements are paramount. This is particularly true when examining the interactions between small molecules and biomolecules/polyelectrolytes, such as DNAs/RNAs, and yet it is overlooked in most publications of thermodynamic binding parameters. This paper presents findings from COST Action 18202 “Network for Equilibria and Chemical Thermodynamics Advanced Research,” which assessed the consistency of data derived from the interactions of calf-thymus DNA (CT-DNA) with the fluorescent intercalator ethidium bromide (EB) through spectrofluorimetric titrations. We first discuss critical experimental aspects and propose a reference experimental protocol which can be used to calibrate procedures for the determination of nucleic acid binding equilibrium constants. We then fit the experimental points according to different procedures and analyse the results focusing on the statistical dispersion of the data, aiming at enlightening the strong and weak points of different fitting procedures. The implications of this work are significant, demonstrating how the statistical dispersion of experimental data can influence the interpretation of biochemical coordination mechanisms. Our study reveals that, despite rigorous protocol standardization, the determination of binding parameters remains sensitive to the choice of data fitting method, with deviations in the logarithmic stability constant (logK) values not falling below 5 % relative standard deviation (RSD), or ± 0.5 logK units for 95 % confidence. This variability evidences the critical need for standardized best practices in data treatment as well as experimental procedures. Although our study focuses on the EB/CT-DNA system through fluorescence titrations, the broader implications for other methodologies across various biochemical systems highlight the importance of this first-of-its-kind inter-laboratory comparison in advancing our understanding of biochemical coordination processes. 2025-02-01T00:00:00Z https://hdl.handle.net/10259/11871 «El obrero de esta Villa es sufrido…»: clase obrera y conflicto social en el este burgalés: Pradoluengo (1820-1936) Martín García, Juan José El proceso de industrialización operado en la localidad burgalesa de Pradoluengo, desde la mecanización de la segunda década del siglo xix hasta el primer tercio del siglo xx, trajo consigo una progresiva bipolarización social y la proletarización de los antiguos fabricantes preindustriales. Con ella, surgieron problemas derivados de la mayor dureza en las condiciones de trabajo, los reducidos salarios y, el más grave de todos, el paro obrero. El sistema laboral a destajo, provocará la carestía o la sobrecarga temporal de trabajo, cubierta con jornales de supervivencia. El obrero pradoluenguino, será condescendiente con su suerte y mostrará escasa capacidad organizativa. La precariedad en la higiene, la inexistencia de medidas de seguridad en las fábricas, la escasa alimentación —causa del elevado número de enfermedades—, y las penosas condiciones de vivienda, contrastan con las opulentas condiciones de vida de los grandes fabricantes y de la burguesía de indianos y comerciantes. Las obras públicas municipales y las limosnas de la oligarquía, maquillan una situación «desagradable» a ojos de estos últimos. Los tímidos intentos de asociacionismo reivindicativo darán sus primeros pasos a finales de los años veinte, siendo cercenados con el comienzo de la Guerra Civil.; The process of industrialisation that tookplace in Pradoluengo (Burgos), from the first mechanisation during the seconddecade of the 19th century until the first third of the 20th, meant gradual butsteady social polarisation, and the proletarianisation of the pre-industrialmanufacturing class. This in turn led to a variety of social problems: the worsening working conditions, lower salariesand irregular work patterns that would oscillate between excessive hours and periodicunemployment. Typically, however, the workers of Pradoluengo seemed to acceptthe situation, and there is little evidence of any capacity for self-organisationwithin the labour force. The poor hygiene, non-existent safety measures in theworkplace, lack of food —the direct cause of many illnesses—, and appallingliving conditions were all in stark contrast to the opulent lifestyles of the newindustrialists, the «indianos» or merchant «bourgeoisie». Municipalworks and the oligarchy’s charity barely disguised a situation that even thislatter class itself found disturbing. However, the first timid moves towardsany form of social protest or labour movements did not emerge until the 1920s, and would come to an abrupt end with the outbreak of the Civil War. 2010-06-01T00:00:00Z https://hdl.handle.net/10259/11870 La consolidación del señorío del monasterio de Cañas en el bajo Najerilla en la Edad Moderna (siglos XV-XVII) Martín García, Juan José Durante la Edad Moderna, el monasterio cisterciense de Santa María de Cañas, reestructurará su dominio en el bajo Najerilla, principalmente en los lugares de Hormilleja y San Pedro de Ruego. Las monjas bernardas recurrirán al pago en dinero de sus rentas, con la entrega de las tierras a censo perpetuo. De facto, estas se convertirán en bienes hereditarios, siempre que los herederos cumplan ciertas condiciones de pago. El juro perpetuo establecerá obligaciones como la vecindad, así como exigencias económicas realmente duras, que provocarán enfrentamientos entre los campesinos y las religiosas, quienes saldrán victoriosas de la mayoría de los pleitos entablados. La escasez de repeticiones en la reproducción del proceso en el interior de las mismas familias, parece indicar una enorme severidad de las cotidianas jornadas de trabajo. Por último, el monasterio conseguirá hacer frente a las pretensiones vecinales o de otros poderes religiosos, sobre el disfrute de las distintas producciones, aunque deberá pagar un alto precio en la compra final de la jurisdicción de abadengo, llevada a cabo en 1678, y de cuyo desembolso, se aprovecharán las exhaustas arcas de la Monarquía.; During the Early Modern period, the Cistercian convent of Santa Maria de Cañas restructured its demesne in the lower Najerilla valley, mainly around the villages of Hormilleja and San Pedro de Ruego. The Bernadine nuns resorted to cash payments, with lands being handed over for perpetual rents. Indeed, such tenancies would become de facto hereditary possessions as long as the successive heirs fulfilled their financial duties. The obligations placed on such tenants included residence as well as genuinely burdensome economic demands which would in turn provoke conflict between villagers and nuns, the latter emerging victorious from the majority of the resulting lawsuits. The lack of repetitions of the process within the same families would seem to point towards the tremendous harshness of everyday working conditions. Finally, the convent successfully resisted pressures from both villagers and other religious groups on the different means of production it controlled, although it would have to pay a high price for abbatial jurisdiction which was finally achieved in 1678 to the benefit of an exhausted royal treasury. 2012-01-01T00:00:00Z https://hdl.handle.net/10259/11869 Los protocolos notariales como fuente para la Historia Contemporánea: economía, política, sociedad y vida cotidiana en la Rioja Alta, en las escribanías de Cerezo y Redecilla (1800-1833) Martín García, Juan José El presente artículo, estudia la potencialidad como fuente para la Historia Contemporánea que suponen los protocolos notariales. En concreto, se analizan los elaborados en escribanías de dos villas hoy burgalesas (Redecilla del Camino y Cerezo de Río Tirón), pero referidos a localidades actualmente riojanas. La periodización abarca el primer tercio del siglo XIX, limitada fundamentalmente por dos acontecimientos relevantes, la Guerra de la Independencia y la muerte de Fernando VII. Los legajos, posibilitan el acercamiento a profundos cambios económicos operados en esta zona rural, como los provocados por exacciones y ventas de bienes concejiles durante la guerra y con posterioridad. Por otro lado, revelan el mantenimiento de la influencia del clero, tanto en la economía como en la política, generalmente proclive a los postulados absolutistas, con la excepción de los curas afrancesados de Hervías. No menos interesantes, son los enfrentamientos y denuncias que traen consigo los giros políticos, y que enmascaran rivalidades familiares, como el caso de Tormantos en 1825. Finalmente, el acercamiento a aspectos de la vida cotidiana, como deslindes, promesas de casamiento, contratos matrimoniales, herencias, etcétera, completan una fotografía de unas comunidades, que comienzan a mutar sus estáticas estructuras antiguorregimentales.; In this article we examine the potential of notarial protocols as source material for Contemporary History. Specifically, we will look at the protocols produced by the scribes of two towns which today are both in Burgos province (Redecilla del Camino and Cerezo de Río Tirón), but which refer to neighbouring villages in the Rioja region. Our study is centred on the first third of the nineteenth-century, a period delimited by two particularly relevant events: the Spanish War of Independence and the death of Ferdinand VII. The protocols allow us a glimpse of the profound economic changes taking place in this rural area, such as levies, special taxes and the sale of municipal property during the war and in its aftermath. They also reveal the continued influence of the clergy, both in economic and political spheres, generally favouring the absolutist cause, though with the notable exception of the French-leaning priests of Hervías. No less interesting are the conflicts and lawsuits which both influence political postures and mask family rivalries, as seen in Tormantos in 1825. Finally, these documents reveal various different aspects of everyday life, recording estate limits, betrothals, marriage contracts, inheritances, etc., and thus offering us a vision of communities just beginning to challenge and change the immemorial structures of the ancien regime. 2014-01-01T00:00:00Z