https://hdl.handle.net/10259/6168 Mon, 12 Oct 2026 15:45:00 GMT 2026-10-12T15:45:00Z https://hdl.handle.net/10259/12253 The Role of Dynamic 3D Models in Evaluating MXenes Toxicity: A Focus on Pulmonary and Hepatic Cells Fuente Vivas, Dalia de la; Parra de la Parra, Sandra de la; Sendra Vega, Marta; Rumbo Lorenzo, Carlos; Martel Martín, Sonia; Barros García, Rocío; Gómez Cuadrado, Laura Background: MXenes and Graphene possess outstanding physicochemical properties. They have strong application potential in electronics, energy storage, and biomedicine. Challenge: Current nanoparticle regulations present critical gaps, particularly in human health risk assessment. There is a growing need for Safe and Sustainable by Design (SSbD) aligned evaluation strategies. Aim: To bridge the in vitro–in vivo gap in nanosafety research. Approach: Advanced in vitro models, including static and dynamic 3D cellular systems, were applied and compared to conventional 2D cultures. Impact: These methodologies offer promising tools for improving the hazard assessment of 2D-nanomaterials, such as MXenes. Póster presentado en: 59th Congress of the European Societies of Toxicology (EUROTOX 2025) "Toxicology addresses society's real life risks for sustainable health and well being", 14-17 September 2025, Athens, Greece Wed, 01 Jan 2025 00:00:00 GMT https://hdl.handle.net/10259/12253 2025-01-01T00:00:00Z https://hdl.handle.net/10259/12250 PEST-PD:The Pathto Early Parkinson´s Detection Preliminary Findings from an Ongoing Longitudinal Observational Study for Prodromal Parkinson’s disease in farmers exposed to pesticides Gómez Cuadrado, Laura; García García, David; Canepa Oneto, Antonio Jesús; Lara, Daniel de; Alonso Núñez, Belén; López Marín, Sofía; Fuente Vivas, Dalia de la; Villanueva Alameda, María Isabel; Barros García, Rocío; Aparicio Martínez, Santiago; Rodríguez Diez, Juan José; López Nozal, Carlos; Ramírez Sanz, José Miguel; Gómez-Gregoris, Irene; Catón, Beatriz; Díaz Hurtado, Jorge; Calvo Simal, Sara; Trigo, Javier; Sánchez, Javier; Lisei, Darío; Olalla Gallo, Mª Ángeles; Allende del Río, Marta; Rivadeneyra Posadas, Jéssica Jannett; Begum, Bacinoglu Merve; Moda, Fabio; Cubo Delgado, Esther PEST-PD is a multi-center project that brings together a multidisciplinary research team composed of neurologists, primary care physicians, physicians specialized in Clinical Analysis and Nuclear Medicine, radiophysicists, biotechnologists, biologists, biostatisticians, agricultural-, telecommunications- and health- engineers, with the aim to understand different aspects of the prevalence of preclinical and prodromal PD in subjects exposed and not exposed to pesticides Póster presentado en: International Congress of Parkinson’s Disease and Movement Disorders, Seoul, Korea, October 4-8, 2026 Thu, 01 Jan 2026 00:00:00 GMT https://hdl.handle.net/10259/12250 2026-01-01T00:00:00Z https://hdl.handle.net/10259/12245 Toxicologial Evaluation of Pristine MXene, PolyPyrrole Functionalized MXene, and MXene-Graphene Hybrids Using Cellular and Computational Models Fuente Vivas, Dalia de la; Huerta Sainz, Sergio de la; Rodriguez-Vinagre, J.; Palacios Santamaría, David; Aparicio Martínez, Santiago; Gómez Cuadrado, Laura Background: MXenes and Graphene possess outstanding physicochemical properties (thermal stability, electrical conductivity). They have strong application potential in biosensors, conductive ink, and EMI shielding. Challenge: Current (nano)particle regulations present critical gaps, particularly in human health risk assessment. There is a growing need for Safe by Design (SbD) aligned evaluation strategies. Aim: Implementing NAMs (in vitro and in silico) to replace animal testing and effectively bridge the in vitro-in vivo gap in safety assessments. Approach: Step-1 SbD was implemented via chemical hazard identification. In vitro NAMs (static and dynamic 3D spheroids) were compared with 2D cultures in a multi-tiered approach integrating toxicological profiles with predictive in silico models for mechanistic cellular analysis. Impact: Enables SbD-driven, animal-free predictive toxicology by integrating advanced 3D NAMs with in silico mechanistic modeling. Póster presentado a: 23 International Congress ESTIV, Maastricht, 2026, 29 june - 2 july Thu, 01 Jan 2026 00:00:00 GMT https://hdl.handle.net/10259/12245 2026-01-01T00:00:00Z https://hdl.handle.net/10259/12244 Scaffold proteins and cytoskeletal dynamics as modulators of Ras-ERK pathway in cancer Fuente Vivas, Dalia de la; Casar, Berta; Crespo, Pedro An overwhelming body of data unquestionably links the Ras-ERK pathway to the upbringing and progression of human malignancies. About 40% of human cancers harbor activating mutations in proteins involved in this signaling cascade, in particular Ras and Braf. In addition to the main proteins of this signaling pathway, there are several regulatory proteins known as scaffold proteins, that modulate the intensity, amplitude and duration of ERK signals. Interestingly, it can propose the scaffold proteins as antitumoral targets. Considering the hypothesis that scaffold proteins interact and regulate the signal transduction through the Ras-ERK pathway in a coordinated manner we try to find out how these interactions are regulated. Ras proteins are distributed in different types of plasma membrane: microdomains and endomembranes. We have previously demonstrated that compartmentalization dictates Ras utilization of effectors, the intensity of its signals and the biological response of the cells. Cell migration is critical for many physiological processes and is often misregulated in developmental disorders and pathological conditions including cancer. MAPK signaling are known regulators of cytoskeletal dynamics during cellular processes such as cell adhesion and migration. Our hypothesis is that localized ERK signaling mediated through recently identified scaffold proteins and its interactions with the cytoskeleton may regulate specific cellular responses. Here we propose that associations among different scaffold proteins and cytoskeleton will add one further degree of regulation for an already tightly regulated cascade and could provide a novel means for manipulating ERK signals, even with therapeutic purposes. Póster presentado en: 41 Congreso de la SEBBM, Santander, 10-13 septiembre 2018 Mon, 01 Jan 2018 00:00:00 GMT https://hdl.handle.net/10259/12244 2018-01-01T00:00:00Z