2026-04-17T12:22:16Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/101032025-01-31T01:05:33Zcom_10259_4313com_10259_5086com_10259_2604col_10259_4314

Arnáiz Alonso, Ana Talavera Mateo, Lucía González Melendi, Pablo Martínez, Manuel Diaz, Isabel Santamaría Fernández, Mª Estrella 2025-01-30T12:22:29Z 2025-01-30T12:22:29Z 2018 http://hdl.handle.net/10259/10103 10.3389/fpls.2018.00986 1664-462X Tetranychus urticae (two-spotted spider mite) is a striking example of polyphagy among herbivores with an extreme record of pesticide resistance and one of the most significant pests in agriculture. The T. urticae genome contains a large number of cysteine- and serine-proteases indicating their importance in the spider mite physiology. This work is focused on the potential role of the Kunitz trypsin inhibitor (KTI) family on plant defense responses against spider mites. The molecular characterization of two of these genes, AtKTI4 and AtKTI5, combined with feeding bioassays using T-DNA insertion lines for both genes was carried out. Spider mite performance assays showed that independent KTI silencing Arabidopsis lines conferred higher susceptibility to T. urticae than WT plants. Additionally, transient overexpression of these inhibitors in Nicotiana benthamiana demonstrated their ability to inhibit not only serine- but also cysteine-proteases, indicating the bifunctional inhibitory role against both types of enzymes. These inhibitory properties could be involved in the modulation of the proteases that participate in the hydrolysis of dietary proteins in the spider mite gut, as well as in other proteolytic processes. This work was supported by projects from Ministerio de Economía y Competitividad of Spain (projects BIO2014-53508-R, 618105-FACCE-Era Net Plus; BIO2017-83472-R). application/pdf eng Frontiers Media Frontiers in Plant Science. 2018, V. 9, 986 https://doi.org/10.3389/fpls.2018.00986 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Plant-herbivore interphase Tetranychus urticae Arabidopsis thaliana Serine protease inhibitors Cysteine protease inhibitors Spider mite digestion Biotecnología Biotechnology Arabidopsis Kunitz Trypsin Inhibitors in Defense Against Spider Mites info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Frontiers in Plant Science 9 986