2026-04-19T17:04:31Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/101032025-01-31T01:05:33Zcom_10259_4313com_10259_5086com_10259_2604col_10259_4314
Arnáiz Alonso, Ana
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600
0000-0002-3842-498X
Talavera Mateo, Lucía
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González Melendi, Pablo
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Martínez, Manuel
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Diaz, Isabel
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600
Santamaría Fernández, Mª Estrella
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600
2025-01-30T12:22:29Z
2025-01-30T12:22:29Z
2018
http://hdl.handle.net/10259/10103
10.3389/fpls.2018.00986
1664-462X
Tetranychus urticae (two-spotted spider mite) is a striking example of polyphagy among herbivores with an extreme record of pesticide resistance and one of the most significant pests in agriculture. The T. urticae genome contains a large number of cysteine- and serine-proteases indicating their importance in the spider mite physiology. This work is focused on the potential role of the Kunitz trypsin inhibitor (KTI) family on plant defense responses against spider mites. The molecular characterization of two of these genes, AtKTI4 and AtKTI5, combined with feeding bioassays using T-DNA insertion lines for both genes was carried out. Spider mite performance assays showed that independent KTI silencing Arabidopsis lines conferred higher susceptibility to T. urticae than WT plants. Additionally, transient overexpression of these inhibitors in Nicotiana benthamiana demonstrated their ability to inhibit not only serine- but also cysteine-proteases, indicating the bifunctional inhibitory role against both types of enzymes. These inhibitory properties could be involved in the modulation of the proteases that participate in the hydrolysis of dietary proteins in the spider mite gut, as well as in other proteolytic processes.
This work was supported by projects from Ministerio de Economía y Competitividad of Spain (projects BIO2014-53508-R, 618105-FACCE-Era Net Plus; BIO2017-83472-R).
application/pdf
eng
Frontiers Media
Frontiers in Plant Science. 2018, V. 9, 986
https://doi.org/10.3389/fpls.2018.00986
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Plant-herbivore interphase
Tetranychus urticae
Arabidopsis thaliana
Serine protease inhibitors
Cysteine protease inhibitors
Spider mite digestion
Biotecnología
Biotechnology
Arabidopsis Kunitz Trypsin Inhibitors in Defense Against Spider Mites
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Frontiers in Plant Science
9
986
THUMBNAIL
Arnaiz-fips_2018.pdf.jpg
Arnaiz-fips_2018.pdf.jpg
IM Thumbnail
image/jpeg
3103
https://riubu.ubu.es/bitstream/10259/10103/4/Arnaiz-fips_2018.pdf.jpg
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LICENSE
license.txt
license.txt
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https://riubu.ubu.es/bitstream/10259/10103/3/license.txt
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CC-LICENSE
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https://riubu.ubu.es/bitstream/10259/10103/2/license_rdf
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ORIGINAL
Arnaiz-fips_2018.pdf
Arnaiz-fips_2018.pdf
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https://riubu.ubu.es/bitstream/10259/10103/1/Arnaiz-fips_2018.pdf
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10259/10103
oai:riubu.ubu.es:10259/10103
2025-01-31 02:05:33.677
Repositorio Institucional de la Universidad de Burgos
bubrep@ubu.es
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