2026-06-01T20:30:36Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/103292025-03-14T01:05:33Zcom_10259.4_2516com_10259_5086com_10259_2604col_10259.4_2517

00925njm 22002777a 4500 dc Vallejo García, Jorge Lucas author Vallejos Calzada, Saúl author Trigo López, Miriam author García Pérez, José Miguel author Busto Núñez, Mª Dolores author 2025-05 Laccases are a group of versatile and green biocatalysts with high efficiency for the degradation of a wide variety of environmental pollutants. However, the feasibility of free laccase applications is hampered by enzyme inactivation due to exposure to denaturing conditions and difficult recovery, which limits reuse and continuous process design, resulting in high costs. Enzyme immobilization technology is a promising way of overcoming these drawbacks. In this work, laccases from two fungal sources, Myceliophthora thermophila and Trametes versicolor, were immobilized for the first time by covalent interaction (azo-linkages) on a film-shaped polyacrylic material. Optimal immobilization parameters were 10 U/mL enzyme load, 1 h and 25 ºC. The resultant immobilized laccases were characterized as a function of pH, temperature, and substrate concentrations (kinetic parameters) and compared with the free enzyme counterpart. The laccase-polymer hybrid (M. thermophila laccase) showed excellent operational and storage stability, retaining 89.8 % of the initial activity after 15 cycles of reuse and 10 days of storage at 4 ºC. Finally, the laccase-polymer films exhibited > 90 % depletion of bisphenol A in water by a dual adsorption-catalysis mechanism. The adsorption capacity ranged from 61.4 % to 30.5 %. GC-MS analysis revealed the degradation products 5,5′-bis-[1-(4-(4-hydroxyphenyl)-1-methyl-ethyl]-biphenyl-2,2′-diol and 3- or 2-methyl-2,3-ditrobenzofuran, as adsorbed compounds, as well as non-degraded bisphenol A. The significance of this research lies in the demonstrated catalytic efficiency of a new, reusable and cost-effective hybrid material for the removal of phenolic contaminants in water. The findings suggest that laccase-polymer hybrids could be a very useful tool for sustainable wastewater treatment. 2352-1864 http://hdl.handle.net/10259/10329 10.1016/j.eti.2025.104093 Acrylic polymer Biodegradation Bisphenol A Laccase Myceliophthora thermophila Trametes versicolor Optimization and stability of a reusable laccase-polymer hybrid film for the removal of bisphenol A in water