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<dc:creator>Barea Gómez, Pedro</dc:creator>
<dc:creator>Illera Gigante, Alba Ester</dc:creator>
<dc:creator>Melgosa Gómez, Rodrigo</dc:creator>
<dc:creator>Benito Román, Oscar</dc:creator>
<dc:creator>Candela Gil, Helena</dc:creator>
<dc:creator>Beltrán Calvo, Sagrario</dc:creator>
<dc:creator>Sanz Díez, Mª Teresa</dc:creator>
<dc:date>2025-08</dc:date>
<dc:description>Water has been explored as a green extraction agent for isoflavone extraction from okara. First, subcritical water (subW) was explored using two different okaras: (1) washed and dried (OKW) and (2) non-pretreated (OKC). Genistein family was the most abundant, with very low quantities in the glycitein family. Kinetic data revealed interconversion of malonyl-glycosides to β-glycosides in subW, with increasing degradation rate constants for all isoflavones with temperature. A maximum of 1229 μg isoflavone/g of dried okara was obtained at 120 °C after 30 min for OKC. Microwave (MAE) and ultrasound (UAE) technologies achieved 72.8 % and 75.4 %, of the yield of subW. Productivity at the maximum was of 41.0, 82.5 and 92.8 μg isoflavone/g dry okara min for subW, MAE, UAE. However, higher fraction of the more bioavailable forms β-glycoside + aglycone was obtained by subW.</dc:description>
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<dc:identifier>http://hdl.handle.net/10259/10445</dc:identifier>
<dc:language>eng</dc:language>
<dc:publisher>Elsevier</dc:publisher>
<dc:title>Green extraction of isoflavones from okara using subcritical water: Kinetics, optimization, and comparison with other water-based sustainable methods</dc:title>
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