2026-04-20T16:11:05Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/110132025-10-30T01:05:42Zcom_10259_4313com_10259_5086com_10259_2604col_10259_4314
Vallejo García, Jorge Lucas
864
600
Trigo López, Miriam
556
600
0000-0001-5948-1230
Ibeas Cortes, Saturnino
293
600
0000-0003-1831-5943
García García, Félix Clemente
201
600
0000-0002-1784-6884
Busto Núñez, Mª Dolores
82
600
0000-0003-1647-7850
Núñez-Carrero, Karina C.
2e9acada-bf27-46d5-aa8a-ee5aae1dce2c
Alonso-Pastor, Luis E.
7fac61cf-119c-42d1-916f-2ea95c8da736
Vallejos Calzada, Saúl
567
600
0000-0001-5522-6574
2025-10-29T12:34:50Z
2025-10-29T12:34:50Z
2025-12
2590-1230
https://hdl.handle.net/10259/11013
10.1016/j.rineng.2025.107880
This work presents a new polymeric material in the form of a film (FLAC) containing immobilized laccase enzyme through diazo bonds, designed for the degradation of indigo carmine dye, which is highly toxic and commonly found in wastewater from the textile industry. The immobilization of the enzyme in the film was characterized by SEM, FT-IR, DSC, TGA and EDXS. The degradation of the dye by FLAC initiates in the presence of a mediator due to the high redox potential of the dye. Six natural mediators (ferulic acid, syringaldazine, guaiacol, eugenol, thymol, and p-coumaric acid) were tested, and complete degradation of the dye was achieved in 180 min, with a mediator concentration of 1 ppm (syringaldazine) and a dye concentration of 10 ppm. A novelty in this study is the short exposure time of the dye-mediator solution to FLAC (15 min) which allowed the degradation process to continue autonomously after the film was removed. Additionally, it was observed that the material was more effective in the presence of textile washing products, achieving over 99 % degradation in 40 min, surpassing its efficacy in distilled water. Regarding reusability, the material retained >90 % of its activity after five cycles of use and washing. Life Cycle Assessment (LCA) and Techno-Economic Analysis (TEA) identified key levers for techno-economic and environmental viability: shorten cycle time (surfactant media), maximise re-use and per-cycle volume/concentration, implement solvent recovery, and energy decarbonization thereby outlining a roadmap to sustainable scale-up.
We gratefully acknowledge the financial support provided by all funders. Author S. Vallejos received Grant PID2023-147301OB-I00 and Grant 3101166576-166576-29-325 funded by MICIU/AEI /10.13039/501100011033 and FEDER, EU. The financial support provided by Fondo Europeo de Desarrollo Regional-European Regional Development Fund (FEDER, ERDF) and Regional Government of Castilla y León -Consejería de Educación, Junta de Castilla y León- (BU025P23) is gratefully acknowledged. This work was supported by the Regional Government of Castilla y León (Junta de Castilla y León) and by the Ministry of Science and Innovation MICIN and the European Union NextGenerationEU PRTR. J. L. Vallejo-García received the grant PRE2021-09812 funded by MCIN/AEI/ 10.13039/501100011033 and by “ESF Investing in your future”. Author Saul Vallejos received grant BG22/00086 funded by Spanish Ministerio de Universidades.
application/pdf
eng
Elsevier
Results in Engineering. 2025, V. 28, 107880
https://doi.org/10.1016/j.rineng.2025.107880
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica, Técnica y de Innovación 2021-2023/PID2023-147301OB-I00/ES/POLIMEROS INTELIGENTES COMO MATERIALES AVANZADOS, SU DISEÑO Y SINTESIS PARA APLICACIONES EN BIOMEDICINA, AGROALIMENTACION Y SOSTENIBILIDAD AMBIENTAL/
info:eu-repo/grantAgreement/Junta de Castilla y León//BU025P23//Polímeros inteligentes. Aplicación en seguridad y control alimentario y biomédico, construcción sostenible y biorremediación (pin)/
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica, Técnica y de Innovación 2021-2023/PRE2021-098123/ES/
info:eu-repo/grantAgreement/MIU/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/BG22%2F00086/ES/
info:eu-repo/grantAgreement/MICIU//3101166576-166576-29-325/ES/
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Laccase
Enzyme immobilization
Indigo carmine
Dyes bioremediation
Smart-polymer
Polymers
LCA
TEA
Biorremediación
Química orgánica
Bioremediation
Chemistry, Organic
Natural mediators for indigo carmine dye removal with immobilized laccase in polyacrylic films
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Results in Engineering
28
107880
THUMBNAIL
Vallejo-re_2025.pdf.jpg
Vallejo-re_2025.pdf.jpg
IM Thumbnail
image/jpeg
4949
https://riubu.ubu.es/bitstream/10259/11013/4/Vallejo-re_2025.pdf.jpg
b27a45fd719417cb57913feb0614f6d9
MD5
4
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
998
https://riubu.ubu.es/bitstream/10259/11013/3/license.txt
aa90105e1c90a1b4fe810649aa967b7d
MD5
3
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
908
https://riubu.ubu.es/bitstream/10259/11013/2/license_rdf
0175ea4a2d4caec4bbcc37e300941108
MD5
2
ORIGINAL
Vallejo-re_2025.pdf
Vallejo-re_2025.pdf
application/pdf
3187265
https://riubu.ubu.es/bitstream/10259/11013/1/Vallejo-re_2025.pdf
9287a8594b6bd5b7ca5db4216c0103b2
MD5
1
10259/11013
oai:riubu.ubu.es:10259/11013
2025-10-30 02:05:42.448
Repositorio Institucional de la Universidad de Burgos
bubrep@ubu.es
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