2026-04-19T17:04:19Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/111292025-12-13T01:05:47Zcom_10259_6168com_10259_5086com_10259_2604col_10259_6169

Campillo Cora, Claudia 86fd990c-fb5f-4760-a94d-513a28ed66c7 600 Soto Gómez, Diego 612dc3df-ac09-49c2-bd91-0ab069082992 600 Arias Estévez, Manuel 34108172-5260-49ce-a671-28f3ce3bd0c1 600 Fernández Calviño, David f9ccd5bf-9ecd-4f68-9634-4301a464ea96 600 2025-12-12T09:53:14Z 2025-12-12T09:53:14Z 2022-09 2073-4395 https://hdl.handle.net/10259/11129 10.3390/agronomy12102280 2073-4395 The assessment of remediation on metal-polluted soils is usually focused on total and/or bioavailable metal content. However, these chemical variables do not provide direct information about reductions in heavy metals pressure on soil microorganisms. We propose the use of bacterial communities to evaluate the efficiency of three remediation techniques: crushed mussel shell (CMS) and pine bark (PB) as soil amendments and EDTA-washing. A soil sample was polluted with different doses of Cu, Ni, and Zn (separately). After 30 days of incubation, the remediation techniques were applied, and bacterial community tolerance to heavy metals determined. If bacterial communities develop tolerance, it is an indicator that the metal is exerting toxicity on them. Soil bacterial communities developed tolerance to Cu, Ni, and Zn in response to metal additions. After remediation, bacterial communities showed decreases in bacterial community tolerance to Cu, Ni, and Zn for all remediation techniques. For Cu and Ni, soil EDTA-washing showed the greatest reduction of bacterial community tolerance to Cu and Ni, respectively, while for Zn the soil amendment with PB was the most effective remediation technique. Thus, bacterial community tolerance to heavy metals successfully detect differences in the effectiveness of the three remediation techniques. This study has been funded by the Spanish Ministry of Economy and Competitiveness through the project CTM2015-73422-JIN (FEDER Funds) David Fernández Calviño holds a Ramón y Cajal contract (RYC-2016-20411) financed by the Spanish Ministry of Economy, Industry and Competitiveness. Diego Soto-Gómez has a post-doctoral contract “Margarita Salas” funded by European Union—NextGenerationEU, and a Seneca Foundation grant for research stays in international centres (21525/EE/21). Claudia Campillo-Cora holds a predoctoral fellowship with Xunta de Galicia (ED401A-2020/084) funded by Consellería de Educación, Universidade e Formación Profesional. application/pdf eng MDPI Agronomy. 2022, V. 12, n- 10, p. 2280-2294 https://doi.org/10.3390/agronomy12102280 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Metal pollution Soil bioremediation Risk assessment Bacterial growth Pollution-Induced Community Tolerance (PICT) Bio-sorbents Soil washing Edafología Química agrícola Soil science Agricultural chemistry Assessment of Polluted Soil Remediation Using Bacterial Community Tolerance to Heavy Metals as an Indicator info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Agronomy 12 10 2280 2294 THUMBNAIL Campillo-agronomy_2022.pdf.jpg Campillo-agronomy_2022.pdf.jpg IM Thumbnail image/jpeg 3442 https://riubu.ubu.es/bitstream/10259/11129/4/Campillo-agronomy_2022.pdf.jpg 0e9680dbfd4548139d81237b455e1ed0 MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 1362 https://riubu.ubu.es/bitstream/10259/11129/3/license.txt 5d013bfa6e473ff0db22cd82a4d71a70 MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 908 https://riubu.ubu.es/bitstream/10259/11129/2/license_rdf 0175ea4a2d4caec4bbcc37e300941108 MD5 2 ORIGINAL Campillo-agronomy_2022.pdf Campillo-agronomy_2022.pdf application/pdf 2347037 https://riubu.ubu.es/bitstream/10259/11129/1/Campillo-agronomy_2022.pdf 8dea48c2fd14f9dad54ea98311b66b9b MD5 1 10259/11129 oai:riubu.ubu.es:10259/11129 2025-12-13 02:05:47.363 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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