2026-08-23T23:33:37Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/112652026-01-23T01:05:32Zcom_10259_4292com_10259_5086com_10259_2604col_10259_4293

00925njm 22002777a 4500 dc García Sánchez, Lourdes author Melero Gil, Beatriz author Diez Mate, Ana Mª author Jaime Moreno, Isabel author Canepa Oneto, Antonio Jesús author Rovira Carballido, Jordi author 2020-03 Campylobacter spp. are the leading causes of bacterial human gastroenteritis worldwide; being poultry farms the main source of infections. In order to obtain information on prevalence and diversity of Campylobacter-infected flocks in the North of Spain, fourteen farms were studied between autumn and spring in 2014 and 2015, respectively. Moreover, virulence genes involved in pathogenicity and antimicrobial resistance were investigated. A survey about preventive hygiene practices at farms was performed to determine the risky practices that could contribute to the presence of Campylobacter in this step of the poultry food chain. Testing the presence of Campylobacter spp. showed 43 % of the farms were positive during autumn, whereas only 31 % were positive in spring. A very high prevalence within-flock was observed (43.1 % to 88.6 %) and C. jejuni was the most prevalent species in both periods. Genotyping by pulsed field gel electrophoresis (PFGE) showed a high heterogeneity among farms (309 isolates clustered into 21 pulsotypes). Virulence genes were present in all C. jejuni isolates while cdtA and cdtC were absent in C. coli. On the contrary, the latter showed higher antimicrobial resistance than C. jejuni. This study suggests that environment might be one of the main sources for Campylobacter transmission, as water supply seemed to be a clear cause of the contamination in a specific farm. However, in other farms other environmental factors contributed to the contamination, confirming the multifactorial origin of Campylobacter colonization in broilers. Therefore, biosecurity measures in farms are crucial to reduce Campylobacter contamination, which may have important implications for human and animal health. 0167-5877 https://hdl.handle.net/10259/11265 10.1016/j.prevetmed.2020.104935 Poultry farms Prevalence PFGE Virulence genes Antibiotic resistance Spain Genotyping, virulence genes and antimicrobial resistance of Campylobacter spp.isolated during two seasonal periods in Spanish poultry farms