2026-08-23T23:34:31Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/112652026-01-23T01:05:32Zcom_10259_4292com_10259_5086com_10259_2604col_10259_4293
Repositorio Institucional de la Universidad de Burgos
author
García Sánchez, Lourdes
author
Melero Gil, Beatriz
author
Diez Mate, Ana Mª
author
Jaime Moreno, Isabel
author
Canepa Oneto, Antonio Jesús
author
Rovira Carballido, Jordi
2026-01-22T11:10:21Z
2026-01-22T11:10:21Z
2020-03
0167-5877
https://hdl.handle.net/10259/11265
10.1016/j.prevetmed.2020.104935
Campylobacter spp. are the leading causes of bacterial human gastroenteritis worldwide; being poultry farms the main source of infections. In order to obtain information on prevalence and diversity of Campylobacter-infected flocks in the North of Spain, fourteen farms were studied between autumn and spring in 2014 and 2015, respectively. Moreover, virulence genes involved in pathogenicity and antimicrobial resistance were investigated. A survey about preventive hygiene practices at farms was performed to determine the risky practices that could contribute to the presence of Campylobacter in this step of the poultry food chain. Testing the presence of Campylobacter spp. showed 43 % of the farms were positive during autumn, whereas only 31 % were positive in spring. A very high prevalence within-flock was observed (43.1 % to 88.6 %) and C. jejuni was the most prevalent species in both periods. Genotyping by pulsed field gel electrophoresis (PFGE) showed a high heterogeneity among farms (309 isolates clustered into 21 pulsotypes). Virulence genes were present in all C. jejuni isolates while cdtA and cdtC were absent in C. coli. On the contrary, the latter showed higher antimicrobial resistance than C. jejuni. This study suggests that environment might be one of the main sources for Campylobacter transmission, as water supply seemed to be a clear cause of the contamination in a specific farm. However, in other farms other environmental factors contributed to the contamination, confirming the multifactorial origin of Campylobacter colonization in broilers. Therefore, biosecurity measures in farms are crucial to reduce Campylobacter contamination, which may have important implications for human and animal health.
eng
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Poultry farms
Prevalence
PFGE
Virulence genes
Antibiotic resistance
Spain
Genotyping, virulence genes and antimicrobial resistance of Campylobacter spp.isolated during two seasonal periods in Spanish poultry farms
info:eu-repo/semantics/article
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Garcia-pvm_2020.pdf