2026-06-19T17:06:29Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/113212026-02-05T01:05:55Zcom_10259_4862com_10259_5086com_10259_2604col_10259_4863
Repositorio Institucional de la Universidad de Burgos
author
Antón Fidalgo, Nuria
author
Mendes, Marta V.
author
Martín, Juan F.
author
Aparicio, Jesús F.
2026-02-04T12:26:27Z
2026-02-04T12:26:27Z
2004-05
0021-9193
https://hdl.handle.net/10259/11321
10.1128/JB.186.9.2567-2575.2004
1098-5530
Sequencing of the DNA region on the left fringe of the pimaricin gene cluster revealed the presence of a 3.6-kb gene, pimR, whose deduced product (1,198 amino acid residues) was found to have amino acid sequence homology with bacterial regulatory proteins. Database comparisons revealed that PimR represents the archetype of a new class of regulators, combining a Streptomyces antibiotic regulatory protein (SARP)-like N-terminal section with a C-terminal half homologous to guanylate cyclases and large ATP-binding regulators of the LuxR family. Gene replacement of pimR from Streptomyces natalensis chromosome results in a complete loss of pimaricin production, suggesting that PimR is a positive regulator of pimaricin biosynthesis. Gene expression analysis by reverse transcriptase PCR (RT-PCR) of the pimaricin gene cluster revealed that S. natalensis ΔPimR shows no expression at all of the cholesterol oxidase-encoding gene pimE, and very low level transcription of the remaining genes of the cluster except for the mutant pimR gene, thus demonstrating that this regulator activates the transcription of all the genes belonging to the pimaricin gene cluster but not its own transcription.
eng
Atribución 4.0 Internacional
Identification of PimR as a Positive Regulator of Pimaricin Biosynthesis in Streptomyces natalensis
info:eu-repo/semantics/article
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URL
https://riubu.ubu.es/bitstream/10259/11321/1/Anton-jb_2004.pdf
File
MD5
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865108
application/pdf
Anton-jb_2004.pdf