2026-04-27T14:06:04Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/113262026-02-05T01:05:50Zcom_10259_11322com_10259_3591com_10259.4_106com_10259_2604col_10259_11323
Repositorio Institucional de la Universidad de Burgos
author
Martínez Alonso, Marta
author
Gandioso, Albert
author
Thibaudeau, Chloé
author
Qin, Xue
author
Arnoux, Philippe
author
Demeubayeva, Nurikamal
author
Guérineau, Vincent
author
Frochot, Céline
author
Jung, Alain C.
author
Gaiddon, Christian
author
Gasser, Gilles
2026-02-04T13:52:34Z
2026-02-04T13:52:34Z
2023-08
1439-4227
https://hdl.handle.net/10259/11326
10.1002/cbic.202300203
1439-7633
A novel Ru(II) cyclometalated photosensitizer (PS), Ru-NH2, for photodynamic therapy (PDT) of formula [Ru(appy)(bphen)2]PF6 (where appy=4-amino-2-phenylpyridine and bphen=bathophenanthroline) and its cetuximab (CTX) bioconjugates, Ru-Mal-CTX and Ru-BAA-CTX (where Mal=maleimide and BAA=benzoylacrylic acid) were synthesised and characterised. The photophysical properties of Ru-NH2 revealed absorption maxima around 580 nm with an absorption up to 725 nm. The generation of singlet oxygen (1O2) upon light irradiation was confirmed with a 1O2 quantum yield of 0.19 in acetonitrile. Preliminary in vitro experiments revealed the Ru-NH2 was nontoxic in the dark in CT-26 and SQ20B cell lines but showed outstanding phototoxicity when irradiated, reaching interesting phototoxicity indexes (PI) >370 at 670 nm, and >150 at 740 nm for CT-26 cells and >50 with NIR light in SQ20B cells. The antibody CTX was successfully attached to the complexes in view of the selective delivery of the PS to cancer cells. Up to four ruthenium fragments were anchored to the antibody (Ab), as confirmed by MALDI-TOF mass spectrometry. Nonetheless, the bioconjugates were not as photoactive as the Ru-NH2 complex.
eng
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Antibody
Bioconjugation
Cetuximab
Metals inmedicine
Photodynamic therapy
Ruthenium
A Novel Near‐IR Absorbing Ruthenium(II) Complex as Photosensitizer for Photodynamic Therapy and its Cetuximab Bioconjugates
info:eu-repo/semantics/article
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URL
https://riubu.ubu.es/bitstream/10259/11326/1/Martinez-ChemBioChem_2023.pdf
File
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4284867
application/pdf
Martinez-ChemBioChem_2023.pdf