2026-06-16T10:56:34Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/113702026-02-17T01:05:41Zcom_10259_11209com_10259_5086com_10259_2604col_10259_11210

Adding Diversity to a Diruthenium Biscyclopentadienyl Scaffold via Alkyne Incorporation: Synthesis and Biological Studies Bresciani, Giulio Boni, Serena Funaioli, Tiziana Zacchini, Stefano Pampaloni, Guido Busto Vázquez, Natalia Biver, Tarita Marchetti, Fabio Antineoplásicos Compuestos organometálicos Antineoplastic agents Organometallic compounds We report the synthesis and the assessment of the anticancer potential of two series of diruthenium biscyclopentadienyl carbonyl complexes. Novel dimetallacyclopentenone compounds (2–4) were obtained (45–92% yields) from the thermal reaction (PhCCPh exchange) of [Ru2Cp2(CO)(μ-CO){μ-η1:η3-C(Ph)═C(Ph)C(═O)}], 1, with alkynes HCCR [R = C5H4FeCp (Fc), 3-C6H4(Asp), 2-naphthyl; Cp = η5-C5H5, Asp = OC(O)-2-C6H4C(O)Me]. Protonation of 1–3 by HBF4 afforded the corresponding μ-alkenyl derivatives 5–7, in 40–86% yields. All products were characterized by IR and NMR spectroscopy; moreover, cyclic voltammetry (1, 2, 5, 7) and single-crystal X-ray diffraction (5, 7) analyses were performed on representative compounds. Complexes 5–7 revealed a cytotoxic activity comparable to that of cisplatin in A549 (lung adenocarcinoma), SW480 (colon adenocarcinoma), and ovarian (A2780) cancer cell lines, and 2, 5, 6, and 7 overcame cisplatin resistance in A2780cis cells. Complexes 2, 5, and 7 (but not the aspirin derivative 6) induced an increase in intracellular ROS levels. Otherwise, 6 strongly stabilizes and elongates natural DNA (from calf thymus, CT-DNA), suggesting a possible intercalation binding mode, whereas 5 is less effective in binding CT-DNA, and 7 is ineffective. This trend is reversed concerning RNA, and in particular, 7 is able to bind poly(rA)poly(rU) showing selectivity for this nucleic acid. Complexes 5–7 can interact with the albumin protein with a thermodynamic signature dominated by hydrophobic interactions. Overall, we show that organometallic species based on the Ru2Cp2(CO)x scaffold (x = 2, 3) are active against cancer cells, with different incorporated fragments influencing the interactions with nucleic acids and the production of ROS. We gratefully acknowledge for financial support La Caixa Foundation (LCF/PR/PR12/11070003), Ministerio de Ciencia, Innovación y Universidades (RTI2018-102040-B-100), Consejería de Educación, Junta de Castilla y León, FEDER (BU305P18), and the University of Pisa (Fondi di Ateneo 2021 and Fondi di Ateneo 2022). This contribution is part of the work from COST Action CA18202, NECTAR − Network for Equilibria and Chemical Thermodynamics Advanced Research, supported by COST (European Cooperation in Science and Technology). CIRCMSB (Consorzio InterUniversitario di Ricerca in Chimica dei Metalli nei Sistemi Biologici) is also acknowledged. 2026-02-16T16:52:26Z 2026-02-16T16:52:26Z 2023-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 0020-1669 https://hdl.handle.net/10259/11370 10.1021/acs.inorgchem.3c01644 1520-510X eng Inorganic Chemistry. 2023, V. 62, n. 31, p. 12453–12467 https://doi.org/10.1021/acs.inorgchem.3c01644 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess application/pdf American Chemical Society