2026-04-19T17:04:01Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/114872026-03-24T01:05:39Zcom_10259_4313com_10259_5086com_10259_2604col_10259_4314
Arnáiz Alonso, Ana
762
600
0000-0002-3842-498X
Melero Gil, Beatriz
356
600
0000-0001-8982-8186
Trigo López, Miriam
556
600
0000-0001-5948-1230
Mendía Jalón, Aránzazu
362
600
0000-0002-2839-465X
Fuente Vivas, Dalia de la
919
600
0000-0001-7037-1251
Iñigo Martínez, María Emilia
972
600
Gómez Cuadrado, Laura
928
600
0000-0002-4754-2582
Ibeas Cortes, Saturnino
293
600
0000-0003-1831-5943
Vallejos Calzada, Saúl
567
600
0000-0001-5522-6574
2026-03-23T09:18:33Z
2026-03-23T09:18:33Z
2026-03
0925-4005
https://hdl.handle.net/10259/11487
10.1016/j.snb.2025.139378
Listeria monocytogenes is a major foodborne pathogen associated with listeriosis, with a mortality rate of up to 30 %. Conventional detection methods are often time-consuming and require sophisticated equipment or complementary techniques to ensure sensitivity and specificity. This study presents a smart chromogenic polymeric sensor for the rapid detection of L. monocytogenes based on the activity of phosphatidylinositol-specific phospholipase C (PI-PLC). Six hydrophilic polymer films with identical compositions but different surface properties were developed, incorporating a chromogenic substrate that produces a visible colour change upon enzymatic hydrolysis, allowing the direct visual identification of L. monocytogenes. The sensitivity and specificity were assessed against a panel of foodborne bacteria, with detection limits of 104 CFUs/mL. Among the tested materials, NC2sf, Ff, and Sf showed the highest sensitivity and limited cross-reactivity with L. ivanovii, Staphylococcus aureus, and Bacillus cereus. Biocompatibility assays in HepG2 cells confirmed acceptable cytocompatibility, underscoring the importance of substrate selection for minimising adverse cellular effects. A proof-of-concept test on chicken breast slices and fresh-cut melon demonstrated the specific detection of L. monocytogenes and clear discrimination from non-pathogenic L. innocua, confirming the robustness and selectivity of the system. Finally, a Life Cycle Assessment highlighted the environmental impact of the sensing materials and provided insights into their potential pre-industrial scalability.
We gratefully acknowledge the financial support provided by all the funders. The financial support provided by Fondo Europeo de Desarrollo Regional-European Regional Development Fund (FEDER, ERDF) and Regional Government of Castilla y León -Consejería de Educación, Junta de Castilla y León- (BU025P23) is gratefully acknowledged. Author S. Vallejos received Grant PID2023–147301OB-I00 and Grant 3101166576–166576–29–325 funded by MICIU/AEI /10.13039/501100011033 and FEDER, EU. This work was supported by the Regional Government of Castilla y León (Junta de Castilla y León) and by the Ministry of Science and Innovation MICIN and the European Union NextGenerationEU PRTR. Author Saul Vallejos received grant BG22/00086 funded by Spanish Ministerio de Universidades.
application/pdf
eng
Elsevier
Sensors and Actuators B: Chemical 2026, V. 451, 139378
https://doi.org/10.1016/j.snb.2025.139378
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Listeria
L. monocytogenes
Polymer-based biosensors
Chromogenic
PI-PLC protease activity
Smart material
Polymeric sensors
Listeriosis
Sensores químicos
Chemical detectors
Naked-eye detection of Listeria monocytogenes using smart chromogenic polymers with tuneable surface morphologies
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Sensors and Actuators B: Chemical
451
139378
THUMBNAIL
Arnaiz-sabc_2026.pdf.jpg
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Arnaiz-sabc_2026.pdf
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oai:riubu.ubu.es:10259/11487
2026-03-24 02:05:39.323
Repositorio Institucional de la Universidad de Burgos
bubrep@ubu.es
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