2026-05-11T17:30:11Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/115982026-05-09T00:05:54Zcom_10259_7463com_10259_5086com_10259_2604col_10259_7464

Gas sensor for 4-ethylguaiacol detection based on tyrosinase enzymatic activity in a deep eutectic solvent Portugal Gómez, Paula Svigelj, Rossella Zanette, Fabiola Toniolo, Rosanna Domínguez Renedo, Olga Alonso Lomillo, Mª Asunción Phenols Screen-printed carbon electrodes Nanomaterials Gas sensor Deep eutectic solvents Wine analysis Vinos-análisis Wine and wine making-Analysis The use of environmentally friendly, cost-effective and biodegradable deep eutectic solvents (DESs) as nonaqueous solvents and electrolytes offers a promising avenue for enhancing enzymatic sensors and extending their applicability to the gas phase. In this study, an assembly is presented that includes a paper crown modified with tyrosinase enzyme, soaked in a DES and positioned on a disposable screen-printed carbon electrode. The paper crown contacts the outer edge of the carbon disk working electrode, as well as the peripheral counter and reference electrodes. This assembly yields a portable and disposable electrochemical platform, effortlessly immobilising DESs onto a porous and economical supporting material like paper. Moreover, the entire configuration resulted in a sensitive, rapidly responsive, membrane-free gas sensor whose response time depended exclusively on the enzymatic reaction. The electroanalytical capabilities of this setup were evaluated through voltammetric and amperometric determinations of phenols in synthetic and real wine samples. The proposed gas enzymatic sensor demonstrated excellent analytical performance for detecting phenolic compounds, offering fast measurement times, simple operation, high sensitivity, wide linear range and good repeatability. This research was supported by PID2023-149522OB-100 funded by MCIU/AEI/https://doi.org/10.13039/501100011033/FEDER,UE, and by the Italian Ministry of University and Research (PON 2014–2020, Action IV.6, 34-G-19900–3, project title “Smart Green Biosensors To Improve Food Sustainability and Environmental Preservation”). Open access funding provided by FEDER European Funds and the Junta de Castilla y León under the Research and Innovation Strategy for Smart Specialization (RIS3) of Castilla y León 2021-2027. 2026-05-08T10:41:02Z 2026-05-08T10:41:02Z 2025-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 0026-3672 https://hdl.handle.net/10259/11598 10.1007/s00604-025-07218-6 1436-5073 eng Microchimica acta. 2025, V. 192, n. 6, 364 https://doi.org/10.1007/s00604-025-07218-6 Atribución 4.0 Internacional http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess application/pdf Springer