2026-09-20T05:33:10Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/121042026-09-18T00:05:32Zcom_10259_4862com_10259_5086com_10259_2604col_10259_4863

Enríquez, Lorena L. 9e6ba96d-4af1-4ea2-93a0-acf48d39edb6 Mendes, Marta V. 205189bc-9aae-42b2-9b18-3552ae187f47 600 Antón Fidalgo, Nuria 993 600 0009-0006-6899-9043 Tunca, Sedef 4271e923-31e9-4a75-8472-f4ff3ca59c83 Guerra, Susana M. 554a89fb-6c13-4da9-8684-d272ec7c4ab1 Martín, Juan F. 0b347238-b881-4aeb-886b-2f4c1a336c7e Aparicio, Jesús F. f95ca308-8c47-4a0b-ac24-52dcf621928d 600 2026-09-17T08:31:02Z 2026-09-17T08:31:02Z 2006-04 0378-1097 https://hdl.handle.net/10259/12104 10.1111/j.1574-6968.2006.00189.x 1574-6968 Streptomyces natalensis produces the antifungal polyene macrolide pimaricin. Genetic manipulation of its biosynthetic genes has been hampered by the lack of efficient gene transfer systems. We have developed a gene transfer system based on intergeneric conjugation from Escherichia coli. Using this approach, we managed to attain transformation efficiencies of 1 × 10⁻⁴ exconjugants per recipient when using self-replicating vectors such as pHZ1358. The use of integrative vectors such as pSET152 or pSOK804 resulted in significantly lower efficiencies. Site-specific integration or the use of self-replicating plasmids did not affect pimaricin production or the essential functions of S. natalensis. Use of DNA methylation proficient E. coli donor strains resulted in no transformants, indicating the presence of methyl-specific restriction systems in S. natalensis. This methodology will enable easier manipulation of the genes responsible for pimaricin biosynthesis, and could prove valuable for the generation of new designer polyene macrolides with better antifungal activity and pharmacological properties. As an example of the validity of the method, we describe the introduction of Supercos-1-derived cosmid vectors into S. natalensis in order to promote gene replacements by double crossover recombination. application/pdf eng Oxford University Press FEMS Microbiology Letters. 2006, v. 257, n. 2, p. 312-318 https://doi.org/10.1111/j.1574-6968.2006.00189.x Conjugation Pimaricin Polyene Streptomyces natalensis Ingeniería genética Genetic engineering An efficient gene transfer system for the pimaricin producer Streptomyces natalensis info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion info:eu-repo/semantics/openAccess FEMS Microbiology Letters 257 2 312 318 THUMBNAIL Anton-fml_2006.pdf.jpg Anton-fml_2006.pdf.jpg IM Thumbnail image/jpeg 4213 https://riubu.ubu.es/bitstream/10259/12104/3/Anton-fml_2006.pdf.jpg 8faf7616e266a3bb6676bf4731aaa0a2 MD5 3 LICENSE license.txt license.txt text/plain; charset=utf-8 999 https://riubu.ubu.es/bitstream/10259/12104/2/license.txt b295bcbce42e2caabeb0c623d3860c06 MD5 2 ORIGINAL Anton-fml_2006.pdf Anton-fml_2006.pdf application/pdf 460678 https://riubu.ubu.es/bitstream/10259/12104/1/Anton-fml_2006.pdf 0cc8f7cc74187e5c785739192184bba2 MD5 1 10259/12104 oai:riubu.ubu.es:10259/12104 2026-09-18 02:05:32.699 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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