2026-10-03T03:00:12Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/121502026-09-26T00:05:34Zcom_10259_4862com_10259_5086com_10259_2604col_10259_4863
Mendes, Marta V.
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600
Recio, Eliseo
aa907dfd-903d-4049-9bda-40b55d2add45
Antón Fidalgo, Nuria
993
600
0009-0006-6899-9043
Guerra, Susana M.
554a89fb-6c13-4da9-8684-d272ec7c4ab1
600
Santos-Aberturas, Javier
fed0398d-ea9f-437d-a892-f387ecb59f68
Martín, Juan F.
0b347238-b881-4aeb-886b-2f4c1a336c7e
600
Aparicio, Jesús F.
f95ca308-8c47-4a0b-ac24-52dcf621928d
600
2026-09-25T10:46:37Z
2026-09-25T10:46:37Z
2007-03
1074-5521
https://hdl.handle.net/10259/12150
10.1016/J.CHEMBIOL.2007.01.010
The gene cluster responsible for pimaricin biosynthesis in Streptomyces natalensis contains a cholesterol oxidase-encoding gene (pimE) surrounded by genes involved in pimaricin production. Gene-inactivation and -complementation experiments revealed that pimE encodes a functional cholesterol oxidase and, surprisingly, that it is also involved in pimaricin biosynthesis. This extracellular enzyme was purified from S. natalensis culture broths to homogeneity, and it was shown to restore pimaricin production when added to the mutant culture broths. Other cholesterol oxidases also triggered pimaricin production, suggesting that these enzymes could act as signaling proteins for polyene biosynthesis. This finding constitutes the description of a cholesterol oxidase gene with an involvement in antibiotic biosynthesis, and it broadens the scope of the biological functions for this type of oxidase.
application/pdf
eng
Cell press
Chemistry & Biology. 2007, V. 14, n. 3, p. 279-290
10.1016/j.chembiol.2007.01.010
Cholesterol oxidases
Signaling proteins
Polyene Macrolide Pimaricin
Genética bacteriana
Antibióticos
Bacterial genetics
Antibiotics
Cholesterol Oxidases Act as Signaling Proteins for the Biosynthesis of the Polyene Macrolide Pimaricin
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
info:eu-repo/semantics/openAccess
Chemistry & Biology
14
3
279
290
THUMBNAIL
Anton-cb_2007.pdf.jpg
Anton-cb_2007.pdf.jpg
IM Thumbnail
image/jpeg
4516
https://riubu.ubu.es/bitstream/10259/12150/3/Anton-cb_2007.pdf.jpg
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MD5
3
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
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https://riubu.ubu.es/bitstream/10259/12150/2/license.txt
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2
ORIGINAL
Anton-cb_2007.pdf
Anton-cb_2007.pdf
application/pdf
861351
https://riubu.ubu.es/bitstream/10259/12150/1/Anton-cb_2007.pdf
6ed9efdfdeced014325376f0d94c94a7
MD5
1
10259/12150
oai:riubu.ubu.es:10259/12150
2026-09-26 02:05:34.648
Repositorio Institucional de la Universidad de Burgos
bubrep@ubu.es
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