2026-10-03T02:56:36Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/122132026-10-03T00:05:23Zcom_10259_4244com_10259_5086com_10259_2604col_10259_4569

Biocascade approach to valorize okara by using subcritical water to obtain valuable compounds: scaling up proces Barea Gómez, Pedro Illera Gigante, Alba Ester Melgosa Gómez, Rodrigo Benito Román, Oscar Candela Gil, Helena Beltrán Calvo, Sagrario Sanz Díez, Mª Teresa Okara Subcritical water Bio-cascade Protein Scale-up Póster presentado en: XVI International Scientific Agriculture Symposium "Agrosym 2025", Jahorina, Bosnia and Herzegovina, October 2-5, 2025 The use of water under subcritical conditions (subW) for the processing of okara, a by-product of soybean beverage production, has promoted advancements in sustainable resource management. A key advantage of using water as a solvent is the elimination of the need for additional chemicals. Industrial okara (Frías Nutrición S.A.U., Burgos, Spain) exhibits a moisture content of 82% w/w and a protein content of 32% w/w. The subW process is environmentally friendly and eliminates the need for a drying step, thus reducing energy costs. Various stages have been proposed to fractionate the valuable bioactive compounds of this by-product: (1) extraction of isoflavones at 120 °C in a batch reactor and (2) extraction and hydrolysis of proteins from the residual solid at higher temperatures in a semi-continuous reactor. More than 1000 μg isoflavones/g dry okara was obtained after 30 min at 120 °C, with the genistein family being the most abundant group. The residual solid was processed in a 2-liter semicontinuous extractor without drying. A total of 625 grams of solid were placed in baskets horizontally. Water flowed at 0.82 L/min, with a residence time of 2.7 minutes. Two sequential temperatures were tested, 180ºC and 270ºC. At 270ºC, approximately 80% of the protein was hydrolyzed. Very low concentrations of degradation products were observed, owing to the short residence time. A medium productivity values of 1.2 g protein/Lh was achieved in a semi-continuous plant. SubW treatment is a promising green chemistry approach for the sustainable valorization of by-products. This work was supported by the Spanish Research Agency (AEI) [grant number PID2022-136385OB-I00], Junta de Castilla y León (JCyL), and European Regional Development Fund (ERDF) [grant number BU027P23]. A.E. Illera postdoctoral contract was funded by BU027P23. H Candela pre-doctoral contract was funded by JCyL and the European Social Fund [ORDEN EDU/1009/2024]. P. Barea pre-doctoral contract is funded by JCyL and the European Social Fund (ESF) [ORDEN EDU/1868/2022]. 2026-10-02T10:24:32Z 2026-10-02T10:24:32Z 2025 info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/acceptedVersion https://hdl.handle.net/10259/12213 eng info:eu-repo/semantics/openAccess application/pdf https://riubu.ubu.es/bitstream/10259/12213/3/Barea-Agrosym_2025.pdf.jpg Hispana TEXT http://rightsstatements.org/vocab/CNE/1.0/ RIUBU. Repositorio Institucional de la Universidad de Burgos https://hdl.handle.net/10259/12213