2026-04-28T02:31:51Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/38182024-07-18T09:40:35Zcom_10259_9433com_10259_5087com_10259_2728col_10259_9434

Arcos Martínez, Julia 36 500 0000-0002-4932-6445 Alonso Lomillo, Mª Asunción 21 500 0000-0002-5714-3956 Iglesias García, Ángela 3724deb4-f5f5-422d-9361-0ab6dd11597b 500 Universidad de Burgos. Departamento de Química cdb9be44-6a4e-4c59-a087-29b53a5cbbc9 500 2015-08-27T10:35:32Z 2015-08-27T10:35:32Z 2014 http://hdl.handle.net/10259/3818 The necessity of disposable biosensors for simple, rapid and inexpensive analysis in fields such as clinical, environmental or industrial has been highlighted over the past decade. In this way, screen-printed electrodes (SPEs) have been shown as inexpensive and reproducible devices for mass production of miniaturized biosensors [1-4]. These transducers, building by sequential layer deposition on the surface of ceramic or plastic substrates and curing steps, have been conventionally linked to the sensing element by adsorption, cross-linking, electropolymerization or covalent bonding. Bioelements are commonly immobilized after the printing and firing processes, because of the high temperatures reached during the curing step [5]. The immobilization procedure requires maintaining the initial properties of the enzyme intact. Thus, successful developments of biosensors largely rely on the cost and stability of the sensing elements [3]. Even if the above-mentioned immobilization procedures are efficient, they imply additional steps after fabrication of the screen-printed carbon electrodes (SPCEs), which extends the whole biosensor manufacturing. Screen-printing techniques also offer another attractive immobilization procedure consisting of printing the biological material. Enzymes, which are proteins able to catalyse specific chemical reactions in vivo, are by far the most commonly employed bioelements [1]. Enzymes can be integrated into the ink to form the sensing paste, which can be screen-printed resulting in biosensors fabricated by only one technology [6-8]. Undoubtedly, this immobilization procedure, which is known as automated immobilization, is particularly interesting for mass production of disposable biosensors. This work presents a simple way for preparing SPCEs modified with Horseradish peroxidase (HRP) for the determination of Levetiracetam (LEV). This second-generation antiepileptic drug (AEDs) has been previously determined using a SPCE-biosensor based on the immobilization of Horseradish peroxidase (HRP) by pyrrole electropolymerization [9] and covalent bonding [10] The screen-printing of HRP-containing ink (SPCHRPEs) offers a higher rapidity and simplicity in the manufacturing process of biosensors for LEV determination. application/pdf spa Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ Este documento está sujeto a una licencia de uso Creative Commons, por la cual está permitido hacer copia, distribuir y comunicar públicamente la obra siempre que se cite al autor original y no se haga de él uso comercial ni obra derivada info:eu-repo/semantics/openAccess Química analítica Chemistry, Analytic Determinación analítica de fármacos con propiedades antiepilépticas info:eu-repo/semantics/bachelorThesis info:eu-repo/semantics/acceptedVersion CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 805 https://riubu.ubu.es/bitstream/10259/3818/8/license_rdf 4460e5956bc1d1639be9ae6146a50347 MD5 8 THUMBNAIL Iglesias_García.pdf.jpg Iglesias_García.pdf.jpg IM Thumbnail image/jpeg 3846 https://riubu.ubu.es/bitstream/10259/3818/7/Iglesias_Garc%c3%ada.pdf.jpg ad77b0e2e487a2e0c70a6f312686e9b1 MD5 7 ORIGINAL Iglesias_García.pdf Iglesias_García.pdf application/pdf 465067 https://riubu.ubu.es/bitstream/10259/3818/1/Iglesias_Garc%c3%ada.pdf a6bbfcaf5bc086b46cfa79a65f96a389 MD5 1 LICENSE license.txt license.txt text/plain; charset=utf-8 1362 https://riubu.ubu.es/bitstream/10259/3818/5/license.txt 5d013bfa6e473ff0db22cd82a4d71a70 MD5 5 TEXT Iglesias_García.pdf.txt Iglesias_García.pdf.txt Extracted text text/plain 44115 https://riubu.ubu.es/bitstream/10259/3818/6/Iglesias_Garc%c3%ada.pdf.txt 43a2fe0277231cdae99ced35f84dd5f3 MD5 6 10259/3818 oai:riubu.ubu.es:10259/3818 2024-07-18 11:40:35.935 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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