2026-05-23T02:05:28Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/42562025-01-20T10:30:27Zcom_10259_4249com_10259_5086com_10259_2604col_10259_4250

Oca Casado, Mª Leticia Rubio Martínez, Laura Ortiz Fernández, Mª Cruz Sarabia Peinador, Luis Antonio García, Inmaculada 2016-10-19T08:29:40Z 2018-02-15T03:45:05Z 2016-02 0169-7439 http://hdl.handle.net/10259/4256 10.1016/j.chemolab.2015.12.019 In this work, the robustness of the sample preparation procedure for the determination of six tranquilizers (xylazine, azaperone, propionylpromazine, chlorpromazine, haloperidol, and azaperol) and a beta-blocker (carazolol) in animal muscle by LC/MS–MS was assessed through the experimental design methodology. A 2III7 − 4 fractional factorial design was performed to evaluate the influence of seven variables on the final concentration of the seven drugs in the samples, in accordance with what is laid down in Commission Decision No 2002/657/EC. The variation considered for each of those seven factors is likely to happen when preparing the samples, being the values chosen as level − 1, the nominal operating conditions. The results of the experimentation were evaluated from different statistical strategies, such as hypothesis testing using an external variance previously estimated, Lenth's method, and Bayesian analysis. Both Lenth's and Bayes' approaches enabled to determine the effect of every variable even though no degrees of freedom were left to estimate the residual error. The same conclusion about the robustness of the extraction step was reached from the three methodologies, namely, none of the seven factors examined influenced on the method performance significantly, so the sample preparation procedure was considered to be robust. Ministerio de Ciencia e Innovación (CTQ2011-26022) and MINECO (CTQ2014- 53157-R). application/pdf eng Elsevier Chemometrics and intelligent laboratory systems. 2016, V. 151, p. 172–180 http://dx.doi.org/10.1016/j.chemolab.2015.12.019 info:eu-repo/grantAgreement/MICINN/CTQ2011-26022 info:eu-repo/grantAgreement/MINECO/CTQ2014- 53157-R Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Robustness Tranquilizer Liquid chromatography–tandem mass spectrometry Commission Decision No 2002/657/EC Lenth's method Bayesian analysis Química analítica Chemistry, Analytic Robustness testing in the determination of seven drugs in animal muscle by liquid chromatography–tandem mass spectrometry info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion