2026-05-23T02:05:04Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/42562025-01-20T10:30:27Zcom_10259_4249com_10259_5086com_10259_2604col_10259_4250
Repositorio Institucional de la Universidad de Burgos
author
Oca Casado, Mª Leticia
author
Rubio Martínez, Laura
author
Ortiz Fernández, Mª Cruz
author
Sarabia Peinador, Luis Antonio
author
García, Inmaculada
2016-10-19T08:29:40Z
2018-02-15T03:45:05Z
2016-02
0169-7439
http://hdl.handle.net/10259/4256
10.1016/j.chemolab.2015.12.019
In this work, the robustness of the sample preparation procedure for the determination of six tranquilizers (xylazine, azaperone, propionylpromazine, chlorpromazine, haloperidol, and azaperol) and a beta-blocker (carazolol) in animal muscle by LC/MS–MS was assessed through the experimental design methodology. A 2III7 − 4 fractional factorial design was performed to evaluate the influence of seven variables on the final concentration of the seven drugs in the samples, in accordance with what is laid down in Commission Decision No 2002/657/EC. The variation considered for each of those seven factors is likely to happen when preparing the samples, being the values chosen as level − 1, the nominal operating conditions. The results of the experimentation were evaluated from different statistical strategies, such as hypothesis testing using an external variance previously estimated, Lenth's method, and Bayesian analysis. Both Lenth's and Bayes' approaches enabled to determine the effect of every variable even though no degrees of freedom were left to estimate the residual error. The same conclusion about the robustness of the extraction step was reached from the three methodologies, namely, none of the seven factors examined influenced on the method performance significantly, so the sample preparation procedure was considered to be robust.
eng
Attribution-NonCommercial-NoDerivatives 4.0 International
Robustness
Tranquilizer
Liquid chromatography–tandem
mass spectrometry
Commission Decision No 2002/657/EC
Lenth's method
Bayesian analysis
Robustness testing in the determination of seven drugs in animal muscle by liquid chromatography–tandem mass spectrometry
info:eu-repo/semantics/article
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