2026-05-06T15:48:10Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/42942022-12-20T09:11:59Zcom_10259_4292com_10259_5086com_10259_2604com_10259.4_2516col_10259_4293col_10259.4_2517

Adaptation and Validation of QUick, Easy, New, CHEap, and Reproducible (QUENCHER) Antioxidant Capacity Assays in Model Products Obtained from Residual Wine Pomace Pino García, Raquel del García Lomillo, Javier Rivero Pérez, Maria Dolores González San José, Mª Luisa Muñiz Rodríguez, Pilar antioxidant activities ABTS Folin−Ciocalteu free radical scavenging assays cellulose Food Biochemistry Molecular biology Alimentos Bioquímica Biología molecular Evaluation of the total antioxidant capacity of solid matrices without extraction steps is a very interesting alternative for food researchers and also for food industries. These methodologies have been denominated QUENCHER from QUick, Easy, New, CHEap, and Reproducible assays. To demonstrate and highlight the validity of QUENCHER (Q) methods, values of Q-method validation were showed for the first time, and they were tested with products of well-known different chemical properties. Furthermore, new QUENCHER assays to measure scavenging capacity against superoxide, hydroxyl, and lipid peroxyl radicals were developed. Calibration models showed good linearity (R2 > 0.995), proportionality and precision (CV < 6.5%), and acceptable detection limits (<20.4 nmol Trolox equiv). The presence of ethanol in the reaction medium gave antioxidant capacity values significantly different from those obtained with water. The dilution of samples with powdered cellulose was discouraged because possible interferences with some of the matrices analyzed may take place. The autonomous government of Castilla y León (Project BU268A11-2) 2016-12-12T11:03:58Z 2016-12-12T11:03:58Z 2015-08 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion 0021-8561 http://hdl.handle.net/10259/4294 eng Journal of Agricultural and Food Chemistry. 2015, V. 63, n. 31, p. 6835-7094 http://dx.doi.org/10.1021/jf5042678 info:eu-repo/semantics/openAccess application/pdf American Chemical Society