2026-06-21T20:32:09Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/43392022-12-19T11:00:07Zcom_10259_4244com_10259_5086com_10259_2604col_10259_4245
Repositorio Institucional de la Universidad de Burgos
author
Melgosa Gómez, Rodrigo
author
Sanz Díez, Mª Teresa
author
García Solaesa, Ángela
author
Bucio López, Silvia Liliana
author
Beltrán Calvo, Sagrario
2017-02-13T11:17:22Z
2017-02-13T11:17:22Z
2015-02
0896-8446
http://hdl.handle.net/10259/4339
This work investigates the effect of supercritical carbon dioxide (SC-CO2) treatment on four commer-cial lipases. The influence of experimental conditions: temperature (35–70◦C), pressure (10–25 MPa),exposure time (1–3 h) and depressurization steps (1–3) on the residual activity was studied. Activityenhancement was verified for free enzymes (Palatase 20000 L and Lipozyme CALB L) treated at mildconditions; while the highest temperature and pressure and the longest exposure time assayed led toactivity losses. On the other hand, activity losses or no significative effect of the treatment in the enzymeactivity were observed on immobilized enzymes (Lipozyme RM IM and Lipozyme 435). Qualitative stud-ies were also performed: Fluorescence emission spectra showed changes in the conformational structureof both two free enzymes after SC-CO2treatment. Scanning electron micrographs showed morphologicalalterations in the immobilization supports of the treated enzymes; while infrared spectra did not showchemical modifications.
eng
Attribution-NonCommercial-NoDerivatives 4.0 International
Candida antarctica lipase B
Rhizomucor miehei lipase
Immobilized lipase
Lipozyme
Enzyme activity
Supercritical carbon dioxide
Enzymatic activity and conformational and morphological studies of four commercial lipases treated with supercritical carbon dioxide
info:eu-repo/semantics/article
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https://riubu.ubu.es/bitstream/10259/4339/1/Melgosa-JSF_2015.pdf
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https://riubu.ubu.es/bitstream/10259/4339/6/Melgosa-JSF_2015.pdf.txt
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Melgosa-JSF_2015.pdf.txt