2026-08-23T14:59:27Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/43592022-04-29T12:02:48Zcom_10259_4313com_10259_5086com_10259_2604com_10259_4365col_10259_4314col_10259_4366

An Organic/Inorganic Hybrid Membrane as a Solid “Turn-On” Fluorescent Chemosensor for Coenzyme A (CoA), Cysteine (Cys), and Glutathione (GSH) in Aqueous Media Vallejos Calzada, Saúl Estévez Bolívar, Pedro Antonio Ibeas Cortes, Saturnino García García, Félix Clemente Serna Arenas, Felipe García Pérez, José Miguel sensory materials chemosensor fluorogenic sensor biomolecules sensing biomolecules The preparation of a fluorogenic sensory material for the detection of biomolecules is described. Strategic functionalisation and copolymerisation of a water insoluble organic sensory molecule with hydrophilic comonomers yielded a crosslinked, water-swellable, easy-to-manipulate solid system for water ‘‘dip-in’’ fluorogenic coenzyme A, cysteine, and glutathione detection by means of host-guest interactions. The sensory material was a membrane with gel-like behaviour, which exhibits a change in fluorescence behaviour upon swelling with a water solution of the target molecules. The membrane follows a “turn-on” pattern, which permits the titration of the abovementioned biomolecules. In this way, the water insoluble sensing motif can be exploited in aqueous media. The sensory motif within the membrane is a chemically anchored piperazinedione-derivative with a weakly bound Hg(II). The response is caused by the displacement of the cation from the membrane due to a stronger complexation with the biomolecules, thus releasing the fluorescent sensory moieties within the membrane 2017-03-14T09:48:16Z 2017-03-14T09:48:16Z 2017-03-14T09:48:16Z 2012-03 info:eu-repo/semantics/article 1424-8220 http://hdl.handle.net/10259/4359 10.3390/s120302969 eng Sensors, 2012, V. 12, n. 3, p. 2969-2982 http://dx.doi.org/10.3390/s120302969 http://creativecommons.org/licenses/by/3.0/ info:eu-repo/semantics/openAccess Attribution 3.0 Unported MDPI