2026-05-07T06:17:04Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/43612022-12-19T13:18:42Zcom_10259_4354com_10259_5086com_10259_2604com_10259_4363com_10259_4365col_10259_4355col_10259_4364col_10259_4366
Chemical speciation of MeHg+ and Hg2+ in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes
Díaz de Greñu Puertas, Borja
García Calvo, José
Cuevas Vicario, José Vicente
García Herbosa, Gabriel
García Ruiz, Begoña
Busto Vázquez, Natalia
Ibeas Cortes, Saturnino
Torroba Pérez, Tomás
Torroba, Blanca
Herrera, Antonio
Pons, Sebastian
Selected new fluorogenic probes that interact in different ways with Hg2+ and MeHg+ have been prepared
and used for the chemical speciation of both cations in aqueous solution as well as in HEK293 cells. The best
selective speciation of Hg2+ and MeHg+ has been achieved by in vitro approaches based on fluorogenic
probes supported in cultured cells, due to the particular sensitivity of the HEK293 cells to permeation by
Hg2+, MeHg+ and the fluorogenic probes. In particular, MeHg+ was selectively detected in cell nuclei by
probe JG45.
2017-03-14
2017-03-14
2015-07
info:eu-repo/semantics/article
http://hdl.handle.net/10259/4361
eng
Chemical Science. 2015, V. 6, n. 7, p. 3757-3764
http://dx.doi.org/10.1039/C5SC00718F
info:eu-repo/grantAgreement/EC/FP7/312411
http://creativecommons.org/licenses/by/3.0/
info:eu-repo/semantics/openAccess
Attribution 3.0 Unported
Royal Society of Chemistry