2026-07-12T15:51:17Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/43782022-04-29T12:02:47Zcom_10259_4376com_10259_5086com_10259_2604col_10259_4377

Barquero Quirós, Miriam Arcos Martínez, Julia 2017-03-17T12:21:52Z 2017-03-17T12:21:52Z 2016-10 1424-8220 http://hdl.handle.net/10259/4378 A novel amperometric biosensor for the determination of Al(III) based on the inhibition of the enzyme superoxide dismutase has been developed. The oxidation signal of epinephrine substrate was affected by the presence of Al(III) ions leading to a decrease in its amperometric current. The immobilization of the enzyme was performed with glutaraldehyde on screen-printed carbon electrodes modifiedwith tetrathiofulvalene (TTF) and different types ofnanoparticles. Nanoparticles of gold, platinum, rhodium and palladium were deposited on screen printed carbon electrodes by means of two electrochemical procedures. Nanoparticles were characterized trough scanning electronic microscopy, X-rays fluorescence, and atomic force microscopy. Palladium nanoparticles showed lower atomic force microscopy parameters and higher slope of aluminum calibration curves and were selected to perform sensor validation. The developed biosensor has a detection limit of 2.0 0.2 Mfor Al(III), with a reproducibility of 7.9% (n = 5). Recovery of standard reference material spiked to buffer solution was 103.8% with a relative standard deviation of 4.8% (n = 5). Recovery of tap water spiked with the standard reference material was 100.5 with a relative standard deviation of 3.4% (n = 3). The study of interfering ions has also been carried out. Vice-presidency for Research at the University of Costa Rica (Project 804-B5-117), Ministerio de Ciencia e Innovación (MICINN Spain) and Fondo Europeo de Desarrollo Regional (FEDER) (Projects :TEC-TEC20013-40561-P and MUSSEL RTC-2015-4077-2). application/pdf eng MDPI Sensors, 2016, V. 16, n. 10, art. 1588 http://dx.doi.org/10.3390/s16101588 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess superoxide dismutase biosensor aluminum tetrathiofulvalene screen-printed electrodes nanoparticles Chemistry, Analytic Química analítica Effect of Nanoparticles on Modified Screen Printed Inhibition Superoxide Dismutase Electrodes for Aluminum info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion