2026-04-28T18:31:58Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/43792022-04-29T12:02:47Zcom_10259_4376com_10259_5086com_10259_2604col_10259_4377

Alvarado Gámez, Ana Lorena d83ef3b7-a53a-4ad5-99b6-f1d3b01baeaa 500 Alonso Lomillo, Mª Asunción 21 500 Domínguez Renedo, Olga 159 500 Arcos Martínez, Julia 36 500 0000-0002-4932-6445 2017-03-17T12:43:01Z 2017-03-17T12:43:01Z 2015-02 http://hdl.handle.net/10259/4379 This paper presents a chronoamperometric method to determine tungsten in water using screen-printed carbon electrodes modified with gold nanoparticles and cross linked alkaline phosphatase immobilized in the working electrode. Enzymatic activity over 2-phospho-L-ascorbic acid trisodium salt, used as substrate, was affected by tungsten ions, which resulted in a decrease of chronoamperometric current, when a potential of 200 mV was applied on 10 mM of substrate in a Tris HCl buffer pH 8.00 and 0.36 M of KCl. Calibration curves for the electrochemical method validation, give a reproducibility of 5.2% (n = 3), a repeatability of 9.4% (n = 3) and a detection limit of 0.29 ± 0.01 μM. Enriched tap water, purified laboratory water and bottled drinking water, with a certified tungsten reference solution traceable to NIST, gave a recovery of 97.1%, 99.1% and 99.1% respectively (n = 4 in each case) and a dynamic range from 0.6 to 30 μM. This study was performed by means of a Lineweaver–Burk plot, showing a mixed kinetic inhibition. Vicerrectorìa de Investigación de la Universidad de Costa Rica (Project 804-B2-297), via Junta de Castilla y León (BU212A12-2), Ministerio de Ciencia e Innovación (MICINN) (TEC2009-12029) and MICINN and Fondo Europeo de Desarrollo Regional (FEDER) (INNPACTO SERIBIO 2011-2014). application/pdf eng MDPI Sensors, 2015, V. 15, n. 2, p. 2232-2243 http://dx.doi.org/10.3390/s150202232 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess alkaline phosphatase chronoamperometry 2-phospho-L-ascorbic acid trisodium salt screen printed carbon electrode tungsten Chemistry, Analytic Química analítica A Chronoamperometric Screen Printed Carbon Biosensor Based on Alkaline Phosphatase Inhibition for W(VI) Determination in Water, Using 2-Phospho-L-Ascorbic Acid Trisodium Salt as a Substrate info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 908 https://riubu.ubu.es/bitstream/10259/4379/8/license_rdf 0175ea4a2d4caec4bbcc37e300941108 MD5 8 THUMBNAIL Alvarado-Sensors_2015.pdf.jpg Alvarado-Sensors_2015.pdf.jpg IM Thumbnail image/jpeg 4532 https://riubu.ubu.es/bitstream/10259/4379/7/Alvarado-Sensors_2015.pdf.jpg a124505a289c50bac0691cc5f9181247 MD5 7 ORIGINAL Alvarado-Sensors_2015.pdf Alvarado-Sensors_2015.pdf application/pdf 721573 https://riubu.ubu.es/bitstream/10259/4379/1/Alvarado-Sensors_2015.pdf c1015596da33a383193cf827b38d9bb6 MD5 1 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/4379/5/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 5 TEXT Alvarado-Sensors_2015.pdf.txt Alvarado-Sensors_2015.pdf.txt Extracted text text/plain 31897 https://riubu.ubu.es/bitstream/10259/4379/6/Alvarado-Sensors_2015.pdf.txt dea2e3bd58dda385999d52c22f488cb3 MD5 6 10259/4379 oai:riubu.ubu.es:10259/4379 2022-04-29 14:02:47.077 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es RWwgYXV0b3IgY29tbyDDum5pY28gdGl0dWxhciBkZSBsb3MgZGVyZWNob3MgZGUgcHJvcGllZGFkIGludGVsZWN0dWFsIGRlIGxhIG9icmEsIG8gZGlzcG9uaWVuZG8gZGUgbG9zIGRlYmlkb3MgcGVybWlzb3MgZGUgbG9zIG90cm9zIHRpdHVsYXJlcywgc2kgbG9zIGh1YmllcmEsIHkgZW4gdmlydHVkIGRlIGxvcyBkZXJlY2hvcyBxdWUgbGUgY29uZmllcmUgbGEgbGVnaXNsYWNpw7NuIHZpZ2VudGUgc29icmUgcHJvcGllZGFkIGludGVsZWN0dWFsIHkgZGVyZWNob3MgZGUgYXV0b3IsIA0KQVVUT1JJWkEgYSBsYSBVbml2ZXJzaWRhZCBkZSBCdXJnb3MgYSBkaWZ1bmRpciwgZGUgbWFuZXJhIGdyYXR1aXRhLCBlbCBjb250ZW5pZG8gZGUgbG9zIGFyY2hpdm9zIGRpZ2l0YWxlcyBxdWUgY29ycmVzcG9uZGVuIGFsIGRvY3VtZW50byBkZXNjcml0byBhbnRlcmlvcm1lbnRlLCBjb24gY2Fyw6FjdGVyIG5vIGV4Y2x1c2l2byB5IGRlIG1hbmVyYSBww7pibGljYSBlbiBhY2Nlc28gYWJpZXJ0byBhIHRyYXbDqXMgZGUgSW50ZXJuZXQsIHBhcmEgbG8gcXVlIGxhIEJpYmxpb3RlY2EgcHJvY2VkZXLDoSBhIGFyY2hpdmFybG9zIGVuIGVsIFJlcG9zaXRvcmlvIEluc3RpdHVjaW9uYWwuIEFzaW1pc21vIGF1dG9yaXphIGEgbGEgVW5pdmVyc2lkYWQgZGUgQnVyZ29zIGEgcmVhbGl6YXIgbGFzIHRyYW5zZm9ybWFjaW9uZXMgbmVjZXNhcmlhcyBkZSBmb3JtYXRvLCBubyBkZSBjb250ZW5pZG8sIHBhcmEgZ2FyYW50aXphciBsYSBwcmVzZXJ2YWNpw7NuIHkgZWwgYWNjZXNvIGVuIGVsIGZ1dHVyby4NCg0KRWwgYXV0b3IgZGlzcG9uZSwgZW4gdG9kbyBjYXNvLCBkZWwgZGVyZWNobyBhIHJldm9jYXIgZXN0YSBhdXRvcml6YWNpw7NuLg0KDQpMYSBjZXNpw7NuIGRlIGRlcmVjaG9zIGRlIGVzdGEgb2JyYSBzZSBlbmN1ZW50cmEgc3VqZXRhIGEgbGEgbGVnaXNsYWNpw7NuIHZpZ2VudGUgc29icmUgcHJvcGllZGFkIGludGVsZWN0dWFsIHkgZGVyZWNob3MgZGUgYXV0b3I=