2026-05-17T04:14:44Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/43812022-04-29T12:02:47Zcom_10259_4376com_10259_5086com_10259_2604col_10259_4377

Alvarado Gámez, Ana Lorena Alonso Lomillo, Mª Asunción Domínguez Renedo, Olga Arcos Martínez, Julia 2017-03-20T09:29:11Z 2017-03-20T09:29:11Z 2014-02 http://hdl.handle.net/10259/4381 A chronoamperometric method for vanadium ion determination, based on the inhibition of the enzyme alkaline phosphatase, is reported. Screen-printed carbon electrodes modified with gold nanoparticles were used as transducers for the immobilization of the enzyme. The enzymatic activity over 4-nitrophenyl phosphate sodium salt is affected by vanadium ions, which results in a decrease in the chronoamperometric current registered. The developed method has a detection limit of 0.39 ± 0.06 µM, a repeatability of 7.7% (n = 4) and a reproducibility of 8% (n = 3). A study of the possible interferences shows that the presence of Mo(VI), Cr(III), Ca(II) and W(VI), may affect vanadium determination at concentration higher than 1.0 mM. The method was successfully applied to the determination of vanadium in spiked tap water Research Vicerrectory of Costa Rica University (Project 804-B0-058) and Spanish Ministry of Science and Innovation (TEC-2009/12029). This work was supported by the Spanish Ministry of Science and Innovation (MICINN) 410 and the European Regional Development Fund (FEDER) (INNPACTO SERIBIO 2011-411 2014) and TEC2009-12029, as well as through Junta de Castilla y León (BU212A12-2). application/pdf eng MDPI Sensors, 2014, V. 14, n. 2, p. 3756-3767 http://dx.doi.org/10.3390/s140203756 Attribution 3.0 Unported http://creativecommons.org/licenses/by/3.0/ info:eu-repo/semantics/openAccess alkaline phosphatase biosensor gold nanoparticles 4-nitrophenyl phosphate screen printed electrode vanadium water analysis Chemistry, Analytic Química analítica A Disposable Alkaline Phosphatase-Based Biosensor for Vanadium Chronoamperometric Determination info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion