2026-05-17T05:21:26Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/43812022-04-29T12:02:47Zcom_10259_4376com_10259_5086com_10259_2604col_10259_4377

00925njm 22002777a 4500 dc Alvarado Gámez, Ana Lorena author Alonso Lomillo, Mª Asunción author Domínguez Renedo, Olga author Arcos Martínez, Julia author 2014-02 A chronoamperometric method for vanadium ion determination, based on the inhibition of the enzyme alkaline phosphatase, is reported. Screen-printed carbon electrodes modified with gold nanoparticles were used as transducers for the immobilization of the enzyme. The enzymatic activity over 4-nitrophenyl phosphate sodium salt is affected by vanadium ions, which results in a decrease in the chronoamperometric current registered. The developed method has a detection limit of 0.39 ± 0.06 µM, a repeatability of 7.7% (n = 4) and a reproducibility of 8% (n = 3). A study of the possible interferences shows that the presence of Mo(VI), Cr(III), Ca(II) and W(VI), may affect vanadium determination at concentration higher than 1.0 mM. The method was successfully applied to the determination of vanadium in spiked tap water http://hdl.handle.net/10259/4381 alkaline phosphatase biosensor gold nanoparticles 4-nitrophenyl phosphate screen printed electrode vanadium water analysis A Disposable Alkaline Phosphatase-Based Biosensor for Vanadium Chronoamperometric Determination