2026-05-17T04:14:25Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/43812022-04-29T12:02:47Zcom_10259_4376com_10259_5086com_10259_2604col_10259_4377
Repositorio Institucional de la Universidad de Burgos
author
Alvarado Gámez, Ana Lorena
author
Alonso Lomillo, Mª Asunción
author
Domínguez Renedo, Olga
author
Arcos Martínez, Julia
2017-03-20T09:29:11Z
2017-03-20T09:29:11Z
2014-02
http://hdl.handle.net/10259/4381
A chronoamperometric method for vanadium ion determination, based on the inhibition of the enzyme alkaline phosphatase, is reported. Screen-printed carbon electrodes modified with gold nanoparticles were used as transducers for the immobilization of the enzyme. The enzymatic activity over 4-nitrophenyl phosphate sodium salt is affected by vanadium ions, which results in a decrease in the chronoamperometric current registered. The developed method has a detection limit of 0.39 ± 0.06 µM, a repeatability of 7.7% (n = 4) and a reproducibility of 8% (n = 3). A study of the possible interferences shows that the presence of Mo(VI), Cr(III), Ca(II) and W(VI), may affect vanadium determination at concentration higher than 1.0 mM. The method was successfully applied to the determination of vanadium in spiked tap water
eng
Attribution 3.0 Unported
alkaline phosphatase
biosensor
gold nanoparticles
4-nitrophenyl phosphate
screen printed electrode
vanadium
water analysis
A Disposable Alkaline Phosphatase-Based Biosensor for Vanadium Chronoamperometric Determination
info:eu-repo/semantics/article
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URL
https://riubu.ubu.es/bitstream/10259/4381/1/Alvarado-Sensors_2014.pdf
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https://riubu.ubu.es/bitstream/10259/4381/6/Alvarado-Sensors_2014.pdf.txt
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