2026-04-28T18:32:04Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/43822022-04-29T12:02:47Zcom_10259_4376com_10259_5086com_10259_2604col_10259_4377

Sanllorente Méndez, Silvia 522 500 Domínguez Renedo, Olga 159 500 Arcos Martínez, Julia 36 500 0000-0002-4932-6445 2017-03-20T10:08:09Z 2017-03-20T10:08:09Z 2010-03 http://hdl.handle.net/10259/4382 Enzymatic amperometric procedures for measuring arsenic, based on the inhibitive action of this metal on acetylcholinesterase enzyme activity, have been developed. Screen-printed carbon electrodes (SPCEs) were used with acetylcholinesterase covalently bonded directly to its surface. The amperometric response of acetylcholinesterase was affected by the presence of arsenic ions, which caused a decrease in the current intensity. The experimental optimum working conditions of pH, substrate concentration and potential applied, were established. Under these conditions, repeatability and reproducibility of biosensors were determined, reaching values below 4% in terms of relative standard deviation. The detection limit obtained for arsenic was 1.1 × 10−8 M for Ach/SPCE biosensor. Analysis of the possible effect of the presence of foreign ions in the solution was performed. The method was applied to determine levels of arsenic in spiked tap water samples. Junta de Castilla y León (BU022A07, Q0968272E) and the Ministerio de Ciencia e Innovación (TEC2008-01367/TEC) is gratefully acknowledged. application/pdf eng MDPI Sensors, 2010, V. 10, n. 3, p. 2119-2128 http://dx.doi.org/10.3390/s100302119 Attribution 3.0 Unported http://creativecommons.org/licenses/by/3.0/ info:eu-repo/semantics/openAccess arsenic determination screen-printed electrode acetylcholinesterase acetylcholinesterase Chemistry, Analytic Química analítica Immobilization of Acetylcholinesterase on Screen-Printed Electrodes. Application to the Determination of Arsenic(III) info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 908 https://riubu.ubu.es/bitstream/10259/4382/8/license_rdf f9a6a4096bf314846151c97900b2090d MD5 8 THUMBNAIL Sanllorente-Sensors_2010.pdf.jpg Sanllorente-Sensors_2010.pdf.jpg IM Thumbnail image/jpeg 4517 https://riubu.ubu.es/bitstream/10259/4382/7/Sanllorente-Sensors_2010.pdf.jpg afb9482a1f5afc6236ebb42ffdf3cb87 MD5 7 ORIGINAL Sanllorente-Sensors_2010.pdf Sanllorente-Sensors_2010.pdf application/pdf 157545 https://riubu.ubu.es/bitstream/10259/4382/1/Sanllorente-Sensors_2010.pdf eeb7eb1c074ef87455d273d42b9dc743 MD5 1 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/4382/5/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 5 TEXT Sanllorente-Sensors_2010.pdf.txt Sanllorente-Sensors_2010.pdf.txt Extracted text text/plain 24807 https://riubu.ubu.es/bitstream/10259/4382/6/Sanllorente-Sensors_2010.pdf.txt 8dd69f856dd2873ad86d406bb128e050 MD5 6 10259/4382 oai:riubu.ubu.es:10259/4382 2022-04-29 14:02:47.123 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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