2026-08-30T06:36:43Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/47242023-10-02T08:19:52Zcom_10259.4_2551com_10259_5086com_10259_2604col_10259.4_2552

Wadsworth, Caroline . Procopio, Noemi . Anderung, Cecilia . Carretero Díaz, José Miguel Iriarte Avilés, Eneko Valdiosera Morales, Cristina Eugenia Rengert Elburg . Kirsty Penkman . Buckley, Michael Ancient DNA Ancient proteins Proteomics Collagen Non-collagenous proteins Ancient DNA (aDNA) is themost informative biomolecule extracted fromskeletal remains at archaeological sites, but its survival is unpredictable and its extraction and analysis is time consuming, expensive and often fails. Several proposed methods for better understanding aDNA survival are based upon the characterisation of some aspect of protein survival, but these are typically non-specific; proteomic analyses may offer an attractive method for understanding preservation processes. In this study, in-depth proteomic (LC-Orbitrap-MS/MS) analyseswere carried out on 69 archaeological bovine bone and dentine samples from multiple European archaeological sites and comparedwith mitochondrial aDNA and amino acid racemisation (AAR) data. Comparisons of these data, including estimations of the relative abundances for seven selected non-collagenous proteins, indicate that the survival of aDNA in bone or dentine may correlatewith the survival of some proteins, and that proteome complexity is a more useful predictor of aDNA survival than protein abundance or AAR. The lack of a strong correlation between the recovery of aDNA and the proteome abundance may indicate that the survival of aDNA is more closely linked to its ability to associate with bone hydroxyapatite crystals rather than to associate with proteins. Significance: Ancient biomolecule survival remains poorly understood, even with great advancements in ‘omics’ technologies, both in genomics and proteomics. This study investigates the survival of ancient DNA in relation to that of proteins, taking into account proteome complexity and the relative protein abundances to improve our understanding of survival mechanisms. The results show that although protein abundance is not necessarily directly related to aDNA survival, proteome complexity appears to be. 2018-02-02 2018-03-01 2017-03 info:eu-repo/semantics/article 1874-3919 http://hdl.handle.net/10259/4724 10.1016/j.jprot.2017.01.004 eng Journal of Proteomics.2017, V. 158, p. 1-8 https://doi.org/10.1016/j.jprot.2017.01.004 info:eu-repo/grantAgreement/MINECO/CGL2012-38434-C03-01 http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 International Elsevier