2026-07-29T11:34:46Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/47762022-12-20T10:27:57Zcom_10259_4354com_10259_5086com_10259_2604com_10259_4365col_10259_4355col_10259_4366

García Calvo, José Ibeas Cortes, Saturnino Antón García, Eva Clara Torroba Pérez, Tomás González Aguilar, Gerardo . Antunes, Wilson . González Lavado, Eloísa . Fanarraga, Mónica L. . 2018-04-10T11:08:35Z 2018-04-10T11:08:35Z 2017-08 2191-1363 http://hdl.handle.net/10259/4776 10.1002/open.201700057 We report the development of new chemical probes for cereulide, a toxic metabolite produced by specific strains of Bacillus cereus, through displacement of potassium cations from a preformed specific complex and a subsequent change in the fluorescence emission. For this purpose, we designed fluorescent probes for potassium cations that were suitable for displacement assays with cereulide from organic extracts. The fluorescence detection of natural cereulide in rice samples was achieved by using synthetic cereulide as a reference and a potassium fluorescent reporter, and this was found to be useful as a portable and fast method for the in situ detection of cereulide in food extracts. To study the fate of cereulide in live cells, we designed a procedure that was suitable for live‐cell microscopy imaging of HeLa cells by comparing the cellular location of the potassium fluorogenic probe, which stained intracellular endolysosomes, in the absence and presence of cereulide; we concluded that in the presence of cereulide, the fluorescence of the probe was decreased because of complexation of the potassium ions by cereulide. Ministerio de Econom&a y Competitividad, Spain (Projects CTQ2015-71353-R and AES-PI16/000496), Junta de Castilla y Lejn, Consejer&a de Educaci jn y Cultura y Fondo Social Europeo (Project BU232U13), and the European Commission, Seventh Framework Programme (Project SNIFFER FP7-SEC-2012–312411) application/pdf eng Wiley-VCH Verlag Chemistry Open. 2017, V. 6, n. 4, p. 562-570 https://doi.org/10.1002/open.201700057 info:eu-repo/grantAgreement/EC/FP7/312411 info:eu-repo/grantAgreement/MINECO/CTQ2015-71353-R info:eu-repo/grantAgreement/MINECO/AES-PI16/000496 info:eu-repo/grantAgreement/JCyL/BU232U13 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Chemistry Química Potassium‐ion‐selective fluorescent sensors to detect cereulide, the emetic toxin of B. cereus, in food samples and HeLa cells info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion