2026-04-28T11:28:29Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/47872022-04-29T12:02:47Zcom_10259_4376com_10259_5086com_10259_2604col_10259_4377
Pires, Filipa Velez
b46b5d5d-25f1-450d-b62a-2335acbdf741
500
Arcos Martínez, Julia
36
500
0000-0002-4932-6445
Cabral, A. Cristina Dias
66ca5a93-94dd-401a-8c70-ba99cb0b13a2
500
Vidal, Juan C. .
2057af1f-2c2b-4147-9529-968f9212cb2a
500
Castillo, Juan R. .
0e0402b0-5154-4ca2-9142-d76f5a7d35bf
500
2018-05-16T08:30:27Z
2018-07
2020-07
0731-7085
http://hdl.handle.net/10259/4787
10.1016/j.jpba.2018.04.019
Human cytomegalovirus (HCMV) is a herpes virus that can cause severe infections. Still, the available methods for its diagnostic have the main disadvantage of requiring long time to be performed. In this work, a simple magnetic particle-based enzyme immunoassay (mpEIA) for the quantification of glycoprotein B of Human cytomegalovirus (gB-HCMV) in urine samples is proposed. The immunosensor scheme is based on the analyte protein gB-HCMV sandwiched between a primary monoclonal antibody, (MBs-PrG-mAb1), and a secondary anti-gB-HCMV antibody labelled with Horseradish peroxidase (Ab2-HRP) to allow spectrophotometric detection.
The mpEIA analytical performance was tested in urine samples, showing a linear dependence between gB-HCMV concentration and the absorbance signal at 450 nm in a range of concentrations from 90 to 700 pg mL−1. The calculated detection limits for gB-HCMV were 90 ± 2 pg mL−1 and the RSD was about 6.7% in urine samples. The immunosensor showed good selectivity against other viruses from Herpesviridae family, namely varicella zoster and Epstein Barr viruses. The recoveries of spiked human urine samples at 0.30–0.50 ng mL−1 concentration levels of gB-HCMV ranged between 91 to 105%. The proposed mpEIA method was validated following the guidelines of the European Medicines Agency (EMEA-2014), and allows rapid, successful and easy quantification of gB-HCMV in urine samples.
FEDER funds through the POCI – COMPETE 2020 – Operational Program Competitiveness and Internationalization in Axis I – Strengthening research, technological development and innovation (Project No. POCI-01-0145-FEDRER-007491), Portuguese Funds by FCT - Foundation for Science and Technology (Project UID/Multi/00709/2013) and Spanish Ministry of Science and Innovation (MICINN), Ministry of Economy and Competitiveness (MINECO) and the European Regional Development Fund (FEDER) (TEC20013-40561-P and MUSSEL RTC-2015-4077-2
application/pdf
eng
Elsevier
Journal of Pharmaceutical and Biomedical Analysis. 2018, V. 156, p. 372-378
https://doi.org/10.1016/j.jpba.2018.04.019
info:eu-repo/grantAgreement/UE/POCI-01-0145-FEDRER-007491
info:eu-repo/grantAgreement/FCT-Foundation for Science and Technology/UID/Multi/00709/2013
info:eu-repo/grantAgreement/MICINN/TEC20013-40561-P
info:eu-repo/grantAgreement/MINECO/RTC-2015-4077-2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Magnetic beads
Immunoassay
mpEIA
Human cytomegalovirus
Glycoprotein B
Chemistry, Analytic
Química analítica
A rapid magnetic particle-based enzyme immunoassay for human cytomegalovirus glycoprotein B quantification
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
805
https://riubu.ubu.es/bitstream/10259/4787/8/license_rdf
4460e5956bc1d1639be9ae6146a50347
MD5
8
THUMBNAIL
Pires-JPBA_2018.pdf.jpg
Pires-JPBA_2018.pdf.jpg
IM Thumbnail
image/jpeg
3701
https://riubu.ubu.es/bitstream/10259/4787/7/Pires-JPBA_2018.pdf.jpg
d0ae6f15043d52e2300783dad28fb8ac
MD5
7
ORIGINAL
Pires-JPBA_2018.pdf
Pires-JPBA_2018.pdf
application/pdf
657583
https://riubu.ubu.es/bitstream/10259/4787/1/Pires-JPBA_2018.pdf
7b73a56d9ad6595a9ac19cbb0021e643
MD5
1
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
998
https://riubu.ubu.es/bitstream/10259/4787/5/license.txt
aa90105e1c90a1b4fe810649aa967b7d
MD5
5
TEXT
Pires-JPBA_2018.pdf.txt
Pires-JPBA_2018.pdf.txt
Extracted text
text/plain
41036
https://riubu.ubu.es/bitstream/10259/4787/6/Pires-JPBA_2018.pdf.txt
f34f37fb93ad03ff977784e536c3dfcd
MD5
6
10259/4787
oai:riubu.ubu.es:10259/4787
2022-04-29 14:02:47.165
Repositorio Institucional de la Universidad de Burgos
bubrep@ubu.es
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