2026-04-19T23:35:55Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/49112021-11-10T09:38:27Zcom_10259_4365com_10259_5086com_10259_2604col_10259_4366

Łęczkowska, Anna . 45902a05-0627-4293-b515-1d7578071261 500 González García, Jorge . a60ffa5f-8b50-451e-abac-dd56a2a4672a 500 Pérez Arnáiz, Cristina 435 500 García Ruiz, Begoña 217 500 White, Andrew J. P. . 40da82db-87c9-4a48-8305-532ec2b8957f 500 Vilar, Ramón . 681fac7f-b96c-49d2-adf9-a058f8563e57 500 2018-09-05T09:24:03Z 2019-08-09T02:45:06Z 2018-08 0947-6539 http://hdl.handle.net/10259/4911 10.1002/chem.201802248 The proposed in vivo formation of G-quadruplex DNA (G4 DNA) in promoter regions of oncogenes and in telomeres has prompted the development of small molecules with high affinity and selectivity for these structures. Herein we report the synthesis of a new di-substituted bipyridine ligand and the corresponding complexes with Ni2+ and VO2+. Both these new complexes have been characterized spectroscopically and by X-ray crystallography. Detailed DNA binding studies of these two complexes, together with three previously reported metal salphen complexes, are presented. Using FRET melting assays, the binding affinity and selectivity of the five metal complexes against six different G4 DNA structures as well as a duplex DNA have been determined. In addition, we present detailed ITC and UV/Vis studies to characterize the interaction of the complexes with human telomeric G4 DNA. Finally, we show via a polymerase stop assay that these complexes are able to stabilize a G4 DNA structure (from the c-Myc oncogene promoter) and halt the activity of Taq polymerase. UK’s Engineering and Physical Sciences Research Council (EPSRC) (grant number: EP/H005285/1) application/pdf eng Wiley-VCH Verlag Chemistry-a european journal. 2018, V. 24, n. 45, p. 11785-11794 https://doi.org/10.1002/chem.201802248 info:eu-repo/grantAgreement/EPSRC/EP/H005285/1 cancer DNA binding heterocycles metal complexes polymerase Química física Chemistry, Physical and theoretical Binding studies of metal–salphen and metal–bipyridine complexes towards G‐Quadruplex DNA info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion info:eu-repo/semantics/openAccess THUMBNAIL Łe˛czkowska-chemistry_2018.pdf.jpg Łe˛czkowska-chemistry_2018.pdf.jpg IM Thumbnail image/jpeg 4859 https://riubu.ubu.es/bitstream/10259/4911/4/%c5%81e%cb%9bczkowska-chemistry_2018.pdf.jpg a7f6968a23ea54cfda67bc6225c8aa83 MD5 4 ORIGINAL Łe˛czkowska-chemistry_2018.pdf Łe˛czkowska-chemistry_2018.pdf application/pdf 2027058 https://riubu.ubu.es/bitstream/10259/4911/1/%c5%81e%cb%9bczkowska-chemistry_2018.pdf e09f03ccd94bf5ad31c565bb553e1aa6 MD5 1 LICENSE license.txt license.txt text/plain; charset=utf-8 999 https://riubu.ubu.es/bitstream/10259/4911/2/license.txt b295bcbce42e2caabeb0c623d3860c06 MD5 2 TEXT Łe˛czkowska-chemistry_2018.pdf.txt Łe˛czkowska-chemistry_2018.pdf.txt Extracted text text/plain 52157 https://riubu.ubu.es/bitstream/10259/4911/3/%c5%81e%cb%9bczkowska-chemistry_2018.pdf.txt dcadf60817ad721e3458bb3a0c7c103a MD5 3 10259/4911 oai:riubu.ubu.es:10259/4911 2021-11-10 10:38:27.885 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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