2026-10-12T21:35:06Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/49742021-11-10T09:38:25Zcom_10259_4725com_10259_5086com_10259_2604col_10259_4726

00925njm 22002777a 4500 dc Mateos, Rosa María . author Jiménez, Gema . author Álvarez Gil, Carmen . author Visiedo, Francisco . author Rivera Rodríguez, Fátima . author Santos Rosendo, Celeste . author Rodríguez Pareja, Antonia . author Perdomo Hernández, Germán M. author Lechuga Sancho, Alfonso . author 2018 Low birth weight increases neonatal morbidity and mortality, and surviving infants have increased risk of metabolic and cardiovascular disturbances later in life, as well as other neurological, psychiatric, and immune complications. A gestational excess of glucocorticoids (GCs) is a well-known cause for fetal growth retardation, but the biological basis for this association remains elusive. Placental growth is closely related to fetal growth. The placenta is the main regulator of nutrient transport to the fetus, resulting from the difference between placental nutrient uptake and the placenta’s own metabolism. The aim of this study was to analyze how excess hydrocortisone affects placental glucose and lipid metabolism. Human placenta explants from term physiological pregnancies were cultured for 18 hours under different hydrocortisone concentrations (2.75, 5.5, and 55mM; 1, 2, and 20mg/ml). Placental glucose and lipid uptake and the metabolic partitioning of fatty acids were quantified by isotopic techniques, and expression of specific glucose transporterGLUT1was quantified bywestern blot.Cell viabilitywas assessed byMTT, immunohistochemistry and caspase activity. We found that excess hydrocortisone impairs glucose uptake and lipoprotein lipase (LPL) activity, coincident with a GC-dose dependent inhibition of fatty acid oxidation and esterification. None of the experimental conditions showed an increased cell death. In conclusion, our results show that GC overexposure exerts a dysfunctional effect on lipid transport and metabolism and glucose uptake in human placental explants. These findings could well be directly related to a reduced placental growth and possibly to a reduced supply of nutrients to the fetus and the consequent fetal growth retardation and metabolic programming. 2314-6133 http://hdl.handle.net/10259/4974 10.1155/2018/5106174 Excess hydrocortisone hampers placental nutrient uptake disrupting cellular metabolism