2026-05-23T13:26:53Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/50442021-11-10T09:38:26Zcom_10259_4249com_10259_5086com_10259_2604col_10259_4250

Rubio Martínez, Laura Sanllorente Méndez, Silvia Sarabia Peinador, Luis Antonio Ortiz Fernández, Mª Cruz 2019-01-08T10:19:51Z 2019-05 2021-05-01 0039-9140 http://hdl.handle.net/10259/5044 10.1016/j.talanta.2018.12.046 The simultaneous determination of two food colorants (cochineal (E-120) and erythrosine (E-127)) was achieved by means of excitation-emission fluorescence matrices and three-way PARAFAC decomposition together with the use of a calibration set that contained binary mixtures of both analytes. In the measured conditions, the amount of cochineal present in the sample affected the fluorescence signal of erythrosine since cochineal caused a quenching effect in the fluorescence of the other food additive. However, the signal of cochineal was not affected by the presence of erythrosine. A calibration line for erythrosine was built for each different concentration level of cochineal. The slopes of these regressions were different depending on the amount of quencher, whereas the intercepts were statistically equal to 0 at a 95% confidence level. The quantification of erythrosine was possible using the regression “amount of cochineal” versus “the slope of the calibration line for erythrosine”. Using this procedure, the mean of the absolute values of the relative errors in prediction for mixtures of both colorants were 5.86% (n = 10) for cochineal and 4.17% (n = 10) for erythrosine. Both analytes were unequivocally identified by the correlation between the pure spectra and the PARAFAC excitation and emission spectral loadings. Pitted cherries in syrup were analyzed. Cochineal and erythrosine were detected in those cherries at a concentration of 185.05 mg kg−1 and 10.76 mg kg−1, respectively. These concentration values were statistically equal to the ones obtained with a HPLC/DAD method. Spanish MINECO (AEI/FEDER, UE) through projects CTQ2014–53157-R and CTQ2017‐88894‐R and by Junta de Castilla y León through project BU012P17 application/pdf eng Elsevier Talanta. 2019, V. 196, p. 153-162 https://doi.org/10.1016/j.talanta.2018.12.046 info:eu-repo/grantAgreement/MINECO/CTQ2014–53157-R info:eu-repo/grantAgreement/MINECO/CTQ2017‐88894‐R info:eu-repo/grantAgreement/JCyL/BU012P17 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Cochinea Erythrosine Excitation-emission fluorescence PARAFAC Quenching effect Cherries Química analítica Chemistry, Analytic Determination of cochineal and erythrosine in cherries in syrup in the presence of quenching effect by means of excitation-emission fluorescence data and three-way PARAFAC decomposition info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion