2026-04-19T15:36:13Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/51752024-07-24T11:42:27Zcom_10259_9476com_10259.4_106com_10259_2604col_10259_9477

Tamayo Ramos, Juan Antonio 547 500 0000-0002-7071-002X Rumbo Lorenzo, Carlos 505 500 0000-0002-5038-0334 Caso, Federica a704067a-4849-4374-9ca2-ec63dd6d0ad2 500 Rinaldi, Antonio a1047f96-8e1c-4705-9f66-7bd1b7085323 500 Garroni, Sebastiano 227 500 0000-0001-7686-6589 Notargiacomo, Andrea 71aa74f7-f836-42fe-8030-c3873f3d1960 500 Romero Santacreu, Lorena 489 600 Cuesta López, Santiago 133 500 0000-0002-7401-3889 2019-11-12T11:34:01Z 2019-11-12T11:34:01Z 2018-09 1944-8244 http://hdl.handle.net/10259/5175 10.1021/acsami.8b07245 1944-8252 Polymeric electrospun fibers are becoming popular in microbial biotechnology because of their exceptional physicochemical characteristics, biodegradability, surface-to-volume ratio, and compatibility with biological systems, which give them a great potential as microbial supports to be used in production processes or environmental applications. In this work, we analyzed and compared the ability of Escherichia coli, Pseudomonas putida, Brevundimonas diminuta, and Sphingobium fuliginis to develop biofilms on different types of polycaprolactone (PCL) microfibers. These bacterial species are relevant in the production of biobased chemicals, enzymes, and proteins for therapeutic use and bioremediation. The obtained results demonstrated that all selected species were able to attach efficiently to the PCL microfibers. Also, the ability of pure cultures of S. fuliginis (former Flavobacterium sp. ATCC 27551, a very relevant strain in the bioremediation of organophosphorus compounds) to form dense biofilms was observed for the first time, opening the possibility of new applications for this microorganism. This material showed to have a high microbial loading capacity, regardless of the mesh density and fiber diameter. A comparative analysis between PCL and polylactic acid (PLA) electrospun microfibers indicated that both surfaces have a similar bacterial loading capacity, but the former material showed higher resistance to microbial degradation than PLA. European Union’s H2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 691095. The contracts of J.A.T.-R. and C.R. were supported by the grant nos. BU079U16 and BU092U16, that were co-financed by Junta de Castilla y León and the European Social Fund. application/pdf eng American Chemical Society ACS Applied Materials and Interfaces. 2018, V. 10, n. 38, p. 32773-32781 https://doi.org/10.1021/acsami.8b07245 info:eu-repo/grantAgreement/JCyL/BU079U16 info:eu-repo/grantAgreement/JCyL/BU092U16 info:eu-repo/grantAgreement/EC/H2020/691095 electrospun polycaprolactone microfibers biofilm bacterial attachment biotechnology Microbiología Materiales Microbiology Materials Analysis of Polycaprolactone Microfibers as Biofilm Carriers for Biotechnologically Relevant Bacteria info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion info:eu-repo/semantics/openAccess TEXT Tamayo-acsami_2018i.pdf.txt Tamayo-acsami_2018i.pdf.txt Extracted text text/plain 56871 https://riubu.ubu.es/bitstream/10259/5175/4/Tamayo-acsami_2018i.pdf.txt ed401a4bcd7e463960dcc081d53663ae MD5 4 THUMBNAIL Tamayo-acsami_2018i.pdf.jpg Tamayo-acsami_2018i.pdf.jpg IM Thumbnail image/jpeg 3817 https://riubu.ubu.es/bitstream/10259/5175/3/Tamayo-acsami_2018i.pdf.jpg b513bb98c4e09b22b67b02d6cc7b740a MD5 3 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/5175/2/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 2 ORIGINAL Tamayo-acsami_2018i.pdf Tamayo-acsami_2018i.pdf application/pdf 1939705 https://riubu.ubu.es/bitstream/10259/5175/1/Tamayo-acsami_2018i.pdf 5b0bfbc859c52895dea566f9af0e29b6 MD5 1 10259/5175 oai:riubu.ubu.es:10259/5175 2024-07-24 13:42:27.734 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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