2026-09-13T23:45:59Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/52162024-07-24T11:45:35Zcom_10259_9476com_10259.4_106com_10259_2604col_10259_9477
Repositorio Institucional de la Universidad de Burgos
author
Domi, Brixhilda
author
Rumbo Lorenzo, Carlos
author
García Tojal, Javier
author
Elena Sima, Livia
author
Negroiu, Gabriela
author
Tamayo Ramos, Juan Antonio
2020-02-19T09:41:56Z
2020-02-19T09:41:56Z
2020-01
http://hdl.handle.net/10259/5216
10.3390/ijms21010205
1422-0067
The ability of commercial monolayer graphene oxide (GO) and graphene oxide nanocolloids (GOC) to interact with different unicellular systems and biomolecules was studied by analyzing the response of human alveolar carcinoma epithelial cells, the yeast Saccharomyces cerevisiae and the bacteria Vibrio fischeri to the presence of different nanoparticle concentrations, and by studying the binding affinity of different microbial enzymes, like the α-l-rhamnosidase enzyme RhaB1 from the bacteria Lactobacillus plantarum and the AbG β-d-glucosidase from Agrobacterium sp. (strain ATCC 21400). An analysis of cytotoxicity on human epithelial cell line A549, S. cerevisiae (colony forming units, ROS induction, genotoxicity) and V. fischeri (luminescence inhibition) cells determined the potential of both nanoparticle types to damage the selected unicellular systems. Also, the protein binding affinity of the graphene derivatives at different oxidation levels was analyzed. The reported results highlight the variability that can exist in terms of toxicological potential and binding affinity depending on the target organism or protein and the selected nanomaterial.
eng
Atribución 4.0 Internacional
Graphene
Unicellular organisms
Toxicity
Binding capacity
ATR-FTIR
TEM
ICP-MS
Interaction analysis of commercial graphene oxide nanoparticles with unicellular systems and biomolecules
info:eu-repo/semantics/article
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https://riubu.ubu.es/bitstream/10259/5216/1/Domi-ijms_2020.pdf
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