2026-05-30T04:54:10Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/53182022-05-30T22:42:07Zcom_10259_3596com_10259_5086com_10259_2604com_10259_4292col_10259_3597col_10259_4293

00925njm 22002777a 4500 dc Alcaraz, Raquel author Muñiz Rodríguez, Pilar author Cavia Saiz, Mónica author Palacios, Óscar author Samper, Katia G. author Gil García, Rubén author Jiménez Pérez, Alondra author García Tojal, Javier author García Girón, Carlos author 2020-05 Colorectal cancer is the third most common type of cancer and has a high incidence in developed countries. At present, specific treatments are being required to allow individualized therapy depending on the molecular alteration on which the drug may act. The aim of this project is to evaluate whether HPTSC and HPTSC* thiosemicarbazones (HPTSC = pyridine-2-carbaldehyde thiosemicarbazone and HPTSC* = pyridine-2-carbaldehyde 4N-methylthiosemicarbazone), and their complexes with different transition metal ions as Cu(II), Fe(III) and Co(III), have antitumor activity in colon cancer cells (HT-29 and SW-480), that have different oncogenic characteristics. Cytotoxicity was evaluated and the involvement of oxidative stress in its mechanism of action was analyzed by quantifying the superoxide dismutase activity, redox state by quantification of the thioredoxin levels and reduced/oxidized glutathione rate and biomolecules damage. The apoptotic effect was evaluated by measurements of the levels of caspase 9 and 3 and the index of histones. All the metal-thiosemicarbazones have antitumor activity mediated by oxidative stress. The HPTSC*-Cu was the compound that showed the best antitumor and apoptotic characteristics for the cell line SW480, that is KRAS gene mutated. 0162-0134 http://hdl.handle.net/10259/5318 10.1016/j.jinorgbio.2020.110993 Cell death Cobalt compounds Colon carcinoma Iron compounds Oxidative stress Thiosemicarbazone Thiosemicarbazone-metal complexes exhibiting cytotoxicity in colon cancer cell lines through oxidative stress