2026-04-19T12:58:46Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/53402022-04-29T22:42:07Zcom_10259_4365com_10259_5086com_10259_2604col_10259_4366

Guarra, Federica bf5e189f-7dc0-468d-ab76-517fa251b284 500 Busto Vázquez, Natalia 83 500 Guerri, Annalisa 6fe0cde4-d1e6-47c4-90d9-b09b93faa033 500 Marchetti, Lorella bd83dbd5-b7a1-4319-9e81-3620a967f808 500 Marzo, Tiziano 4a8c372b-5c87-44c3-8d8a-d96991dae66b 500 García Ruiz, Begoña 217 500 Biver, Tarita 686e3c46-a69c-4fbe-9b7a-e05265bbac4e 500 Gabbiani, Chiara c695aa22-3fbb-4bb0-9968-993ee894ad80 500 2020-06-10T12:29:12Z 2020-06-10T12:29:12Z 2020-04 0162-0134 http://hdl.handle.net/10259/5340 10.1016/j.jinorgbio.2020.110998 A silver(I) and a gold(I) complex of the fluorescent N-heterocyclic carbenic (NHC) ligand 1-(9-anthracenylmethyl)-3-(3-trimethylsilyl-2-propynil)-benzimidazol-2-ylidene have been synthesized and characterized. These compounds show cytotoxicity in the micromolar range and higher antiproliferative properties than cisplatin (CDDP) against several tumour cell lines such as SW480 (colon), A549 (lung) and HepG2 (liver). Both metal complexes are successfully internalized by SW480 cells being the silver compound the most accumulated. Subsequently, they were evaluated as inhibitors of the selenoenzyme Thioredoxin reductase (TrxR) and as DNA binders. Fluorescence microscopy confirmed that both protein and DNA binding could be involved in the biological activity of the compounds. The silver carbene was the most effective enzyme inhibitor with an IC50 in the nanomolar range. Also, interaction studies with natural double stranded DNA highlight a strong stabilisation of the double helix after binding to the Ag(I) carbene, indicating its potential suitability as dual-targeting anticancer active molecule. “la Caixa” Foundation (LCF/PR/PR12/11070003), Ministerio de Ciencia Innovación y Universidades-FEDER (RTI2018-102040-B-100) and Junta de Castilla y León-FEDER (BU305P18). application/pdf eng Elsevier Journal of Inorganic Biochemistry. 2020, V. 205, 110998 https://doi.org/10.1016/j.jinorgbio.2020.110998 info:eu-repo/grantAgreement/MICINN/RTI2018-102040-B-100 info:eu-repo/grantAgreement/JCyL/BU305P18 info:eu-repo/grantAgreement/FundaciónLaCaixa/LCF/PR/PR12/11070003 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Dual anticancer drug Metal carbene complexes Thioredoxin reductase Anthracenyl dyes DNA interactions Bioquímica Biochemistry Cytotoxic Ag(I) and Au(I) NHC-carbenes bind DNA and show TrxR inhibition info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Journal of Inorganic Biochemistry 205 110998 TEXT Guarra-jib_2020.pdf.txt Guarra-jib_2020.pdf.txt Extracted text text/plain 70905 https://riubu.ubu.es/bitstream/10259/5340/5/Guarra-jib_2020.pdf.txt e85f219c0e4d924a698ebc2a1c6d11fa MD5 5 THUMBNAIL Guarra-jib_2020.pdf.jpg Guarra-jib_2020.pdf.jpg IM Thumbnail image/jpeg 4467 https://riubu.ubu.es/bitstream/10259/5340/4/Guarra-jib_2020.pdf.jpg 1fd413d6b94f0654834361b86c27a472 MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 999 https://riubu.ubu.es/bitstream/10259/5340/3/license.txt b295bcbce42e2caabeb0c623d3860c06 MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 805 https://riubu.ubu.es/bitstream/10259/5340/2/license_rdf 4460e5956bc1d1639be9ae6146a50347 MD5 2 ORIGINAL Guarra-jib_2020.pdf Guarra-jib_2020.pdf application/pdf 1637393 https://riubu.ubu.es/bitstream/10259/5340/1/Guarra-jib_2020.pdf e435f8de07fd84f1d80af37b921cda04 MD5 1 10259/5340 oai:riubu.ubu.es:10259/5340 2022-04-30 00:42:07.173 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es RWwgYXV0b3IgY29tbyDDum5pY28gdGl0dWxhciBkZSBsb3MgZGVyZWNob3MgZGUgcHJvcGllZGFkIGludGVsZWN0dWFsIGRlIGxhIG9icmEsIG8gZGlzcG9uaWVuZG8gZGUgbG9zIGRlYmlkb3MgcGVybWlzb3MgZGUgbG9zIG90cm9zIHRpdHVsYXJlcywgc2kgbG9zIGh1YmllcmEsIHkgZW4gdmlydHVkIGRlIGxvcyBkZXJlY2hvcyBxdWUgbGUgY29uZmllcmUgbGEgbGVnaXNsYWNpw7NuIHZpZ2VudGUgc29icmUgcHJvcGllZGFkIGludGVsZWN0dWFsIHkgZGVyZWNob3MgZGUgYXV0b3IsIA0KQVVUT1JJWkEgYSBsYSBVbml2ZXJzaWRhZCBkZSBCdXJnb3MgYSBkaWZ1bmRpciwgZGUgbWFuZXJhIGdyYXR1aXRhLCBlbCBjb250ZW5pZG8gZGUgbG9zIGFyY2hpdm9zIGRpZ2l0YWxlcyBxdWUgY29ycmVzcG9uZGVuIGFsIGRvY3VtZW50byBkZXNjcml0byBhbnRlcmlvcm1lbnRlLCBjb24gY2Fyw6FjdGVyIG5vIGV4Y2x1c2l2byB5IGRlIG1hbmVyYSBww7pibGljYSBlbiBhY2Nlc28gYWJpZXJ0byBhIHRyYXbDqXMgZGUgSW50ZXJuZXQsIHBhcmEgbG8gcXVlIGxhIEJpYmxpb3RlY2EgcHJvY2VkZXLDoSBhIGFyY2hpdmFybG9zIGVuIGVsIFJlcG9zaXRvcmlvIEluc3RpdHVjaW9uYWwuIEFzaW1pc21vIGF1dG9yaXphIGEgbGEgVW5pdmVyc2lkYWQgZGUgQnVyZ29zIGEgcmVhbGl6YXIgbGFzIHRyYW5zZm9ybWFjaW9uZXMgbmVjZXNhcmlhcyBkZSBmb3JtYXRvLCBubyBkZSBjb250ZW5pZG8sIHBhcmEgZ2FyYW50aXphciBsYSBwcmVzZXJ2YWNpw7NuIHkgZWwgYWNjZXNvIGVuIGVsIGZ1dHVyby4NCg0KRWwgYXV0b3IgZGlzcG9uZSwgZW4gdG9kbyBjYXNvLCBkZWwgZGVyZWNobyBhIHJldm9jYXIgZXN0YSBhdXRvcml6YWNpw7NuLg0KDQpMYSBjZXNpw7NuIGRlIGRlcmVjaG9zIGRlIGVzdGEgb2JyYSBzZSBlbmN1ZW50cmEgc3VqZXRhIGEgbGEgbGVnaXNsYWNpw7NuIHZpZ2VudGUgc29icmUgcHJvcGllZGFkIGludGVsZWN0dWFsIHkgZGVyZWNob3MgZGUgYXV0b3Iu