2026-04-28T18:32:07Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/53482022-04-08T10:34:38Zcom_10259_4376com_10259_5086com_10259_2604col_10259_4377

Cunha Silva, Hugo 1 500 Pires, Filipa Velez b46b5d5d-25f1-450d-b62a-2335acbdf741 500 Cabral, A. Cristina Dias 66ca5a93-94dd-401a-8c70-ba99cb0b13a2 500 Arcos Martínez, Julia 36 500 0000-0002-4932-6445 2020-06-18T08:42:57Z 2020-06-18T08:42:57Z 2019-12 0927-7765 http://hdl.handle.net/10259/5348 10.1016/j.colsurfb.2019.110490 Lactate oxidase (LOx), recognized to selectively catalyze the lactate oxidation in complex matrices, has been highlighted as preferable biorecognition element for the development of lactate biosensors. In a previous work, we have demonstrated that LOx crosslinking on a modified screen-printed electrode results in a dual range lactate biosensor, with one of the analysis linear range (4 to 50 mM) compatible with lactate sweat levels. It was advanced that such behavior results from an atypical substrate inhibition process. To understand such inhibition phenomena, this work relies in the study of LOx structure when submitted to increased substrate concentrations. The results found by fluorescence spectroscopy and dynamic light scattering of LOx solutions, evidenced conformational changes of the enzyme, occurring in presence of inhibitory substrate concentrations. Therefore, the inhibition behavior found at the biosensor, is an outcome of LOx structural alterations as result of a pH-dependent mechanism promoted at high substrate concentrations. Spanish Ministry of Science and Innovation (MICINN), Ministry of Economy and Competitiveness (MINECO) and the European Regional Development Fund (FEDER) (TEC20013-40561-P and MUSSEL RTC-2015-4077-2). Hugo Cunha-Silva would like to acknowledge funding from the Spanish Ministry of Economy (BES-2014-068214) application/pdf eng Elsevier Colloids and Surfaces B: Biointerfaces. 2019, V. 184, 110490 https://doi.org/10.1016/j.colsurfb.2019.110490 info:eu-repo/grantAgreement/MINECO/MUSSEL RTC-2015-4077-2 info:eu-repo/grantAgreement/MICINN/TEC20013-40561-P Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Lactate oxidase inhibition Amperometric biosensor Lactic acid Fluorescence spectrometry Dynamic light scattering Química analítica Chemistry, Analytic Inhibited enzymatic reaction of crosslinked lactate oxidase through a pH-dependent mechanism info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Colloids and Surfaces B: Biointerfaces 184 110490 TEXT Cunha-csbb_2019.pdf.txt Cunha-csbb_2019.pdf.txt Extracted text text/plain 49981 https://riubu.ubu.es/bitstream/10259/5348/5/Cunha-csbb_2019.pdf.txt b348c2f3517adcb3fde02c1bf0b819e4 MD5 5 THUMBNAIL Cunha-csbb_2019.pdf.jpg Cunha-csbb_2019.pdf.jpg IM Thumbnail image/jpeg 3711 https://riubu.ubu.es/bitstream/10259/5348/4/Cunha-csbb_2019.pdf.jpg 580f9244072f226a1f9369786f03bfa6 MD5 4 LICENSE license.txt license.txt text/plain; charset=utf-8 998 https://riubu.ubu.es/bitstream/10259/5348/3/license.txt aa90105e1c90a1b4fe810649aa967b7d MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 805 https://riubu.ubu.es/bitstream/10259/5348/2/license_rdf 4460e5956bc1d1639be9ae6146a50347 MD5 2 ORIGINAL Cunha-csbb_2019.pdf Cunha-csbb_2019.pdf application/pdf 778477 https://riubu.ubu.es/bitstream/10259/5348/1/Cunha-csbb_2019.pdf e45d324c1db02119d95c1a6fc1199669 MD5 1 10259/5348 oai:riubu.ubu.es:10259/5348 2022-04-08 12:34:38.872 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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