2026-10-12T17:19:00Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/53652021-11-02T12:02:54Zcom_10259_5363com_10259_5086com_10259_2604col_10259_5364

00925njm 22002777a 4500 dc Gargouri, Wafa author Osés Gómez, Sandra María author Fernández Muiño, Miguel Ángel author Sancho Ortiz, María Teresa author Kechaou, Nabil author 2019-08 The aim of this study was to investigate the phytochemical composition and biological properties of Tunisian propolis from four different regions: Kasserine, Béja, Kèf and Monastir. Ethanolic extracts of propolis were prepared using two extraction methods; solvent and ultrasonic extraction. Total phenolics, flavonoids, ABTS free radical and hydroxyl radicals scavenging abilities, anti-inflammatory, anti-hypertensive, as well as antimicrobial activities of propolis extracts were determined. Identification and quantification of phenolic and flavonoid compounds were performed by using both HPLC-UV and HPLC-ESI-MS. The results revealed high contents of total phenolics and flavonoids and polyphenols extraction was more efficient by sonication. Caffeic acid phenethyl ester (CAPE), galangin, and genistein were the major identified compounds. Antihypertensive activity, evaluated in propolis extracts for first time by HPLC-UV, was higher than 90% for all extracts. Tunisian propolis is an important natural source of polyphenols and flavonoids. The best extraction method was ultrasonic for antioxidants and most of biological activities; conventional method seems to be more suitable for anti-inflammatory activity. Propolis from Béja contains the highest amount of antioxidants and have a stronger potential biological activities. Tunisian propolis could be, therefore, a promising raw material for food and pharmaceutical industry. 0023-6438 http://hdl.handle.net/10259/5365 10.1016/j.lwt.2019.05.044 Propolis Phenolics HPLC-UV Antioxidant activity ACE Evaluation of bioactive compounds and biological activities of Tunisian propolis