2026-07-24T18:48:36Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/53672021-11-02T12:02:52Zcom_10259_5363com_10259_5086com_10259_2604col_10259_5364

Phenolic Profile, Antioxidant Capacities and Enzymatic Inhibitory Activities of Propolis from Different Geographical Areas: Needs for Analytical Harmonization Osés Gómez, Sandra María Marcos, Patricia Azofra, Patricia Pablo, Ana de Fernández Muiño, Miguel Ángel Sancho Ortiz, María Teresa propolis phenolic profile antioxidant capacities hyaluronidase inhibitory activity ACE inhibitory activity Propolis is a resinous vegetal exudate modified by bees, and is interesting as a preservative and potentially functional product. This work dealt with studying the common phenolic profiles and antioxidant capacities of 13 bee propolis from different geographical areas. Both hyaluronidase and angiotensin converting enzyme (ACE) inhibitory activities were also assessed and related when possible with particular phenolic compounds. High performance liquid chromatography‐ultraviolet detection (HPLC‐UV) analysis showed that every propolis contained p‐coumaric acid (1.2–12.2 mg/g) and ferulic acid (0.3–11.0 mg/g). Pinocembrin, catechin, and caffeic acid phenethyl ester (CAPE) plus galangin were the main flavonoids. Antioxidant activities were higher than 280 μmol trolox/g for trolox equivalent antioxidant capacity (TEAC), 0.099 mmol uric acid/g for radical‐scavenging effect on hydroxyl radicals, and 0.19 mg/mL for half maximal inhibitory concentration (IC50) of antioxidant activity against superoxide anion radical. Working with solutions of 10 mg/mL propolis, hyaluronidase inhibitory activity ranged between 0% and 68.20%, being correlated to ferulic acid content. ACE inhibitory effect determined by HPLC was higher than 78%, being correlated with catechin and p‐coumaric acid. Therefore, propolis could be useful for food, pharmaceutical, and cosmetic companies, also helping to reduce risk factors for diseases related to oxidative damage, inflammatory processes, and hypertension. This research also highlights the necessity for harmonized analysis methods and the expression of results for propolis. 2020-07-02T10:31:26Z 2020-07-02T10:31:26Z 2020-07-02T10:31:26Z 2020-01 info:eu-repo/semantics/article http://hdl.handle.net/10259/5367 10.3390/antiox9010075 2076-3921 eng Antioxidants. 2020, V. 9, n. 1, 75 https://doi.org/10.3390/antiox9010075 http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Atribución 4.0 Internacional MDPI