2026-05-11T15:40:54Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/54752022-11-30T23:42:08Zcom_10259_4365com_10259_5086com_10259_2604col_10259_4366

Macii, Francesca Pérez Arnáiz, Cristina Arrico, Lorenzo Busto Vázquez, Natalia García Ruiz, Begoña Biver, Tarita 2020-09-22T11:01:45Z 2020-09-22T11:01:45Z 2020-11 0162-0134 http://hdl.handle.net/10259/5475 10.1016/j.jinorgbio.2020.111199 This work concerns an analysis of the binding mechanism of a copper phthalocyanine (Alcian Blue-tetrakis(methylpyridinium) chloride, ABTP) to natural calf thymus DNA, G-quadruplexes (G4) and synthetic RNA polynucleotides in the form of double polyriboadenylic·polyribouridylic acid (poly(A)·poly(U)) or triple strands polyriboadenylic·2polyribouridylic acid (poly(A)·2poly(U)). ABTP is a well know dye that might undergo novel applications, but its interaction with DNA is scarcely studied and we lack information on possible RNA or G4 binding. This might be related to system complexity due to the presence of supramolecular dye-dye aggregates. Despite this, we show here that apparent parameters can be calculated, which provide information on the binding mechanism. Absorbance titrations in the presence of biosubstrate excess, melting and circular dichroism experiments show that ABTP binds to both RNAs and DNA. External/groove binding is the main feature for RNAs, whereas partial intercalation is the major binging mode for DNA. ABTP externally binds to both hybrid, parallel and anti-parallel G4s but seem to show a slightly different binding mode and a preference for anti-parallel structures. The thermodynamic features of the different systems are also discussed in the frame of the enthalpy-entropy compensation phenomenon. la Caixa” Foundation (LCF/PR/PR12/11070003), Ministerio de Ciencia, Innovación y Universidades-FEDER (RTI2018- 102040-B-100) and Junta de Castilla y León-FEDER (BU305P18) application/pdf eng Elsevier Journal of Inorganic Biochemistry. 2020, V. 212, 111199 https://doi.org/10.1016/j.jinorgbio.2020.111199 info:eu-repo/grantAgreement/FundaciónLaCaixa/LCF/PR/PR12/11070003 info:eu-repo/grantAgreement/MICINN/RTI2018- 102040-B-100 info:eu-repo/grantAgreement/JCyL/U305P18 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Groove binding Partial intercalation Helix conformation Enthalpy-entropy compensation Anti-parallel Hybrid Química inorgánica Bioquímica Chemistry, Inorganic Biochemistry Alcian blue pyridine variant interaction with DNA and RNA polynucleotides and G-quadruplexes: changes in the binding features for different biosubstrates info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Journal of Inorganic Biochemistry 212 111199