2026-05-14T06:46:16Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/54752022-11-30T23:42:08Zcom_10259_4365com_10259_5086com_10259_2604col_10259_4366
Repositorio Institucional de la Universidad de Burgos
author
Macii, Francesca
author
Pérez Arnáiz, Cristina
author
Arrico, Lorenzo
author
Busto Vázquez, Natalia
author
García Ruiz, Begoña
author
Biver, Tarita
2020-09-22T11:01:45Z
2020-09-22T11:01:45Z
2020-11
0162-0134
http://hdl.handle.net/10259/5475
10.1016/j.jinorgbio.2020.111199
This work concerns an analysis of the binding mechanism of a copper phthalocyanine (Alcian Blue-tetrakis(methylpyridinium) chloride, ABTP) to natural calf thymus DNA, G-quadruplexes (G4) and synthetic RNA polynucleotides in the form of double polyriboadenylic·polyribouridylic acid (poly(A)·poly(U)) or triple strands polyriboadenylic·2polyribouridylic acid (poly(A)·2poly(U)). ABTP is a well know dye that might undergo novel applications, but its interaction with DNA is scarcely studied and we lack information on possible RNA or G4 binding. This might be related to system complexity due to the presence of supramolecular dye-dye aggregates. Despite this, we show here that apparent parameters can be calculated, which provide information on the binding mechanism. Absorbance titrations in the presence of biosubstrate excess, melting and circular dichroism experiments show that ABTP binds to both RNAs and DNA. External/groove binding is the main feature for RNAs, whereas partial intercalation is the major binging mode for DNA. ABTP externally binds to both hybrid, parallel and anti-parallel G4s but seem to show a slightly different binding mode and a preference for anti-parallel structures. The thermodynamic features of the different systems are also discussed in the frame of the enthalpy-entropy compensation phenomenon.
eng
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Groove binding
Partial intercalation
Helix conformation
Enthalpy-entropy compensation
Anti-parallel
Hybrid
Alcian blue pyridine variant interaction with DNA and RNA polynucleotides and G-quadruplexes: changes in the binding features for different biosubstrates
info:eu-repo/semantics/article
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https://riubu.ubu.es/bitstream/10259/5475/1/Macii-jib-2020.pdf
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https://riubu.ubu.es/bitstream/10259/5475/5/Macii-jib-2020.pdf.txt
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