2026-10-23T13:53:11Zhttps://riubu.ubu.es/oai/requestoai:riubu.ubu.es:10259/55772021-11-02T12:00:41Zcom_10259_5827com_10259_5086com_10259_2604col_10259_5828
Repositorio Institucional de la Universidad de Burgos
author
Gerardi, Gisela
author
Casali, Cecilia I.
author
Cavia Saiz, Mónica
author
Rivero Pérez, Maria Dolores
author
Perazzo, Cecilia
author
González San José, Mª Luisa
author
Muñiz Rodríguez, Pilar
author
Fernández Tome, María C.
2020-12-11T08:37:22Z
2020-12-11T08:37:22Z
2020-11
2405-8440
http://hdl.handle.net/10259/5577
10.1016/j.heliyon.2020.e05396
The functional renal epithelium is composed of differentiated and polarized tubular cells with a strong actin cortex and specialized cell-cell junctions. If, under pathological conditions, these cells have to resist higher kidney osmolarity, they need to activate diverse mechanisms to survive external nephrotoxic agents such as inflammation and oxidative stress. Wine pomace polyphenols exert protective effects on renal cells. In this study, two wine-pomace products and their protective effects upon promotion and preservation of normal cell differentiation and attenuation of oxalate-induced type II epithelial mesenchymal transition (EMT) are evaluated. Treatment with gastrointestinal and colonic bioavailable fractions from red (rWPP) and white (wWPP) wine pomaces, both in the presence and the absence of oxalate, showed similar cell numbers and nuclear size than the non-treated differentiated MDCK cells. Immunofluorescence analysis showed the reduction of morphological changes and the preservation of cellular junctions for the rWPP and wWPP pre-treatment of cells exposed to oxalate injury. Hence, both rWPP and wWPP attenuated oxalate type II EMT in MDCK cells that conserved their epithelial morphology and cellular junctions through the antioxidant activities of grape pomace polyphenols.
eng
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Food science
Cell biology
Plant products
Cell differentiation
Cytoskeleton
Natural product
Polyphenol
Antioxidant
Cancer research
Renal system
Wine pomace
Polyphenols
EMT
Oxalate
E-cadherin
Bioavailable wine pomace attenuates oxalate-induced type II epithelial mesenchymal transition and preserve the differentiated phenotype of renal MDCK cells
info:eu-repo/semantics/article
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URL
https://riubu.ubu.es/bitstream/10259/5577/1/Gerardi-heliyon_2020.pdf
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Gerardi-heliyon_2020.pdf
URL
https://riubu.ubu.es/bitstream/10259/5577/5/Gerardi-heliyon_2020.pdf.txt
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Gerardi-heliyon_2020.pdf.txt