2026-04-20T07:17:41Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/55772021-11-02T12:00:41Zcom_10259_5827com_10259_5086com_10259_2604col_10259_5828

Gerardi, Gisela 229 500 Casali, Cecilia I. c0a20aa4-af5b-4f30-a74b-cb187c826e50 500 Cavia Saiz, Mónica 116 500 Rivero Pérez, Maria Dolores 473 500 Perazzo, Cecilia 9a66d8d5-ff28-4811-8403-129fd0ffdae2 500 González San José, Mª Luisa 258 500 Muñiz Rodríguez, Pilar 380 500 Fernández Tome, María C. 9e954889-dae5-4683-9689-028ac5ba00e5 500 2020-12-11T08:37:22Z 2020-12-11T08:37:22Z 2020-11 2405-8440 http://hdl.handle.net/10259/5577 10.1016/j.heliyon.2020.e05396 The functional renal epithelium is composed of differentiated and polarized tubular cells with a strong actin cortex and specialized cell-cell junctions. If, under pathological conditions, these cells have to resist higher kidney osmolarity, they need to activate diverse mechanisms to survive external nephrotoxic agents such as inflammation and oxidative stress. Wine pomace polyphenols exert protective effects on renal cells. In this study, two wine-pomace products and their protective effects upon promotion and preservation of normal cell differentiation and attenuation of oxalate-induced type II epithelial mesenchymal transition (EMT) are evaluated. Treatment with gastrointestinal and colonic bioavailable fractions from red (rWPP) and white (wWPP) wine pomaces, both in the presence and the absence of oxalate, showed similar cell numbers and nuclear size than the non-treated differentiated MDCK cells. Immunofluorescence analysis showed the reduction of morphological changes and the preservation of cellular junctions for the rWPP and wWPP pre-treatment of cells exposed to oxalate injury. Hence, both rWPP and wWPP attenuated oxalate type II EMT in MDCK cells that conserved their epithelial morphology and cellular junctions through the antioxidant activities of grape pomace polyphenols. Agencia Nacional de Promoci on Científica y Tecnol ogica (PICT 2016-1055), the University of Buenos Aires (UBACYT 2014-2017, 20020130100658BA) and the Ministerio de Ciencia, Innovaci on y Universidades (PGC2018-097113B100). application/pdf eng Elsevier Heliyon. 2020, V. 6, n. 11, e05396 https://doi.org/10.1016/j.heliyon.2020.e05396 info:eu-repo/grantAgreement/MICINN/PGC2018-097113B100 info:eu-repo/grantAgreement/UBA/UBACYT-2014-2017-20020130100658B info:eu-repo/grantAgreement/Agencia Nacional de Promoción Científica y Tecnológica/PICT 2016-1055 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Food science Cell biology Plant products Cell differentiation Cytoskeleton Natural product Polyphenol Antioxidant Cancer research Renal system Wine pomace Polyphenols EMT Oxalate E-cadherin Alimentos Biología molecular Bioquímica Food Molecular biology Biochemistry Bioavailable wine pomace attenuates oxalate-induced type II epithelial mesenchymal transition and preserve the differentiated phenotype of renal MDCK cells info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Heliyon 6 11 e05396 ORIGINAL Gerardi-heliyon_2020.pdf Gerardi-heliyon_2020.pdf application/pdf 3461532 https://riubu.ubu.es/bitstream/10259/5577/1/Gerardi-heliyon_2020.pdf 1e185d346e28bcadcdbee21db9ca0d29 MD5 1 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 805 https://riubu.ubu.es/bitstream/10259/5577/2/license_rdf 4460e5956bc1d1639be9ae6146a50347 MD5 2 LICENSE license.txt license.txt text/plain; charset=utf-8 999 https://riubu.ubu.es/bitstream/10259/5577/3/license.txt b295bcbce42e2caabeb0c623d3860c06 MD5 3 THUMBNAIL Gerardi-heliyon_2020.pdf.jpg Gerardi-heliyon_2020.pdf.jpg IM Thumbnail image/jpeg 5167 https://riubu.ubu.es/bitstream/10259/5577/4/Gerardi-heliyon_2020.pdf.jpg 3ca24fd732aa1ac56f7f9635026a71cf MD5 4 TEXT Gerardi-heliyon_2020.pdf.txt Gerardi-heliyon_2020.pdf.txt Extracted text text/plain 55748 https://riubu.ubu.es/bitstream/10259/5577/5/Gerardi-heliyon_2020.pdf.txt c905f3c3ba44fc32e6e40454fcc13191 MD5 5 10259/5577 oai:riubu.ubu.es:10259/5577 2021-11-02 13:00:41.289 Repositorio Institucional de la Universidad de Burgos bubrep@ubu.es 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