2026-06-22T13:54:27Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/57842024-07-25T07:31:53Zcom_10259_9476com_10259.4_106com_10259_2604col_10259_9477

00925njm 22002777a 4500 dc Schindler, Torben author González, Adrián author Boopathi, Ramachandran author Marty Roda, Marta author Romero Santacreu, Lorena author Wildes, Andew R. author Porcar, Lionel author Martel, Anne author Theodorakopoulos, Nikos author Cuesta López, Santiago author Angelov, Dimitar author Unruh, Tobias author Peyrard, Michel author 2018-10 DNA is a flexible molecule, but the degree of its flexibility is subject to debate. The commonly-accepted persistence length of lp ≈ 500Å is inconsistent with recent studies on short-chain DNA that show much greater flexibility but do not probe its origin. We have performed x-ray and neutron small-angle scattering on a short DNA sequence containing a strong nucleosome positioning element and analyzed the results using a modified Kratky-Porod model to determine possible conformations. Our results support a hypothesis from Crick and Klug in 1975 that some DNA sequences in solution can have sharp kinks, potentially resolving the discrepancy. Our conclusions are supported by measurements on a radiation-damaged sample, where single-strand breaks lead to increased flexibility and by an analysis of data from another sequence, which does not have kinks, but where our method can detect a locally enhanced flexibility due to an AT domain. 2470-0045 http://hdl.handle.net/10259/5784 10.1103/PhysRevE.98.042417 2470-0053 Kinky DNA in solution: Small-angle-scattering study of a nucleosome positioning sequence