2026-06-21T17:43:47Zhttps://riubu.ubu.es/oai/request

oai:riubu.ubu.es:10259/61192022-11-15T13:51:45Zcom_10259_3844com_10259_5086com_10259_2604col_10259_3845

Vulcano, Fabio Kovtun, Alessandro Bettini, Cristian Xia, Zhenyuan Liscio, Andrea Terzi, Fabio Heras Vidaurre, Aránzazu Colina Santamaría, Álvaro Zanfrognini, Barbara Melucci, Manuela 2021-11-08T12:02:02Z 2021-11-08T12:02:02Z 2020-04 2053-1583 http://hdl.handle.net/10259/6119 10.1088/2053-1583/ab734f 2053-1583 We describe a nanocomposite material for the electrochemical detection of β-nicotinamide adenine dinucleotide (NADH), a coenzyme involved in redox reactions of all living cells and in the detection of many organic species by electrochemical biosensors. The composite is made of nanosheets of electrochemically exfoliated graphene oxide (EGO) covalently functionalized with dopamine (DP) molecules. The EGODP material finally obtained is rich of 1,2-dihydroxyphenyl moieties and is able to detect NADH at a particular low potential value with higher sensitivity with respect to pristine EGO. To study the effectiveness of 1,2-dihydroxyphenyl moieties in inducing electrocatalytic oxidation of NADH, we combined standard voltammetric techniques with UV–Vis absorption spectroelectrochemistry, which allowed us to measure the variations in composition occurring at the electrode|solution interface, i.e. to measure the consumption rate of NADH. Spectroelectrochemical tests performed by polarising the electrode at a fixed potential value were finally used to compare the performance of EGODP with both EGO and EGO-DP blend (MIX) for the detection of NADH. The covalently functionalized EGO (EGODP) shows sensitivity to NADH up to 300 M−1, around 180 % and 140 % better than either pristine EGO or MIX, respectively. Spanish Ministerio de Economía y Competitividad (Grants CTQ2017-83935-R-AEI/FEDERUE), Ministerio de Ciencia, Innovación y Universidades (RED2018-102412-T) and Junta de Castilla y León (Grant BU297P18). application/pdf spa IOP Publishing 2D Materials. 2020, V. 7, n. 2, 024007 https://doi.org/10.1088/2053-1583/ab734f info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2017-83935-R/ES/ESPECTROELECTROQUIMICA RAMAN CUANTITATIVA info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/ RED2018-102412-T/ES/RED DE SENSORES Y BIOSENSORES ELECTROQUIMICOS info:eu-repo/grantAgreement/Junta de Castilla y León//BU297P18//Fusión de técnicas espectroelectroquímicas avanzadas Attribution-NonCommercial-NoDerivs 3.0 Unported http://creativecommons.org/licenses/by-nc-nd/3.0/ info:eu-repo/semantics/openAccess graphene oxide spectroelectrochemistry chemical functionalization 1,2-dihydroxyphenyl moieties NADH Química analítica Chemistry, Analytic Dopamine-functionalized graphene oxide as a high-performance material for biosensing info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion